Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress | |
Xu, Daorong1,2; Qian, Jikun1,2; Guan, Xin1,2; Ren, Ling3; Yang, Kaifan1,2; Huang, Xuan4; Zhang, Shuyuan3; Chai, Yu1,2; Wu, Xiaohu1,2; Wu, Hangtian1,2 | |
刊名 | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY |
2021-01-14 | |
卷号 | 8页码:12 |
关键词 | 316-5Cu stainless steel type H vessel M2a macrophage PDGF-BB immunoregulation |
ISSN号 | 2296-4185 |
DOI | 10.3389/fbioe.2020.620629 |
通讯作者 | Zhang, Xianrong(xianrongzh@smu.edu.cn) ; Yang, Ke(kyang@imr.ac.cn) ; Yu, Bin(yubin@smu.edu.cn) |
英文摘要 | In the mammalian skeletal system, osteogenesis and angiogenesis are closely linked by type H vessels during bone regeneration and repair. Our previous studies confirmed the promotion of these processes by copper-containing metal (CCM) in vitro and in vivo. However, whether and how the coupling of angiogenesis and osteogenesis participates in the promotion of bone regeneration by CCM in vivo is unknown. In this study, M2a macrophages but not M2c macrophages were shown to be immunoregulated by CCM. A CCM, 316L-5Cu, was applied to drilling hole injuries of the tibia of C57/6 mice for comparison. We observed advanced formation of cortical bone and type H vessels beneath the new bone in the 316L-5Cu group 14 and 21 days postinjury. Moreover, the recruitment of CD206-positive M2a macrophages, which are regarded as the primary source of platelet-derived growth factor type BB (PDGF-BB), was significantly promoted at the injury site at days 14 and 21. Under the stimulation of CCM, mitochondria-derived reactive oxygen species were also found to be upregulated in CD206(hi) M2a macrophages in vitro, and this upregulation was correlated with the expression of PDGF-BB. In conclusion, our results indicate that CCM promotes the evolution of callus through the generation of type H vessels during the process of bone repair by upregulating the expression of PDGF-BB derived from M2a macrophages. |
资助项目 | Major Program of National Natural Science Foundation of China[81830079] ; National Natural Science Foundation of China[31870954] ; National Natural Science Foundation of China[81702009] |
WOS研究方向 | Biotechnology & Applied Microbiology ; Science & Technology - Other Topics |
语种 | 英语 |
出版者 | FRONTIERS MEDIA SA |
WOS记录号 | WOS:000615949700001 |
资助机构 | Major Program of National Natural Science Foundation of China ; National Natural Science Foundation of China |
内容类型 | 期刊论文 |
源URL | [http://ir.imr.ac.cn/handle/321006/159162] |
专题 | 金属研究所_中国科学院金属研究所 |
通讯作者 | Zhang, Xianrong; Yang, Ke; Yu, Bin |
作者单位 | 1.Southern Med Univ, Nanfang Hosp, Div Orthoped Surg, Dept Orthoped, Guangzhou, Peoples R China 2.Southern Med Univ, Nanfang Hosp, Guangdong Prov Key Lab Bone & Cartilage Regenerat, Guangzhou, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang, Peoples R China 4.Southern Med Univ, Nanfang Hosp, Dept Infect Dis, State Key Lab Organ Failure Res,Guangdong Prov Ke, Guangzhou, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Daorong,Qian, Jikun,Guan, Xin,et al. Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2021,8:12. |
APA | Xu, Daorong.,Qian, Jikun.,Guan, Xin.,Ren, Ling.,Yang, Kaifan.,...&Yu, Bin.(2021).Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8,12. |
MLA | Xu, Daorong,et al."Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2021):12. |
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