Role of placenta-specific protein 1 in trophoblast invasion and migration

doi:10.1530/REP-14-0052

CORC > 金属研究所 > 中国科学院金属研究所

	Role of placenta-specific protein 1 in trophoblast invasion and migration
	Chang, Wen-Lin 1,2; Yang, Qing 1; Zhang, Hui ; Lin, Hai-Yan 2,3; Zhou, Zhi 2,4; Lu, Xiaoyin 2,4; Zhu, Cheng 2; Xue, Li-Qun 1; Wang, Hongmei 2
刊名	REPRODUCTION
	2014-10-01
卷号	148 期号:4 页码:343-352
ISSN号	1470-1626
DOI	10.1530/REP-14-0052
通讯作者	Wang, Hongmei(wanghm@ioz.ac.cn)
英文摘要	Placenta-specific protein 1 (PLAC1), a placenta-specific gene, is known to be involved in the development of placenta in both humans and mice. However, the precise role of PLAC1 in placental trophoblast function remains unclear. In this study, the localization of PLAC1 in human placental tissues and its physiological significance in trophoblast invasion and migration are investigated by technical studies including real-time RT-PCR, in situ hybridization, immunohistochemistry, and functional studies by utilizing cell invasion and migration assays in the trophoblast cell line HTR8/SVneo as well as the primary inducing extravillous trophoblasts (EVTs). The results show that PLAC1 is mainly detected in the trophoblast columns and syncytiotrophoblast of the first-trimester human placental villi, as well as in the EVTs that invade into the maternal decidua. Knockdown of PLAC1 by RNA interference significantly suppresses the invasion and migration of HTR8/SVneo cells and shortens the distance of the outgrowth of the induced EVTs from the cytotrophoblast column of the explants. All the above data suggests that PLAC1 plays an important role in human placental trophoblast invasion and migration.
资助项目	National Key Basic Research Program of China[2011CB944400] ; National Key Basic Research Program of China[2012CB126310] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA01020102] ; Natural Science Foundation of China[81225004] ; Natural Science Foundation of China[31271603] ; Natural Science Foundation of China[31171438]
WOS研究方向	Developmental Biology ; Reproductive Biology
语种	英语
出版者	BIOSCIENTIFICA LTD
WOS记录号	WOS:000343331100004
资助机构	National Key Basic Research Program of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/125871]
专题	金属研究所_中国科学院金属研究所
通讯作者	Wang, Hongmei
作者单位	1.Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China 2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China 3.Capital Med Univ, Beijing Obstet & Gynecol Hosp, Beijing 100069, Peoples R China 4.Grad Sch Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Chang, Wen-Lin,Yang, Qing,Zhang, Hui,et al. Role of placenta-specific protein 1 in trophoblast invasion and migration[J]. REPRODUCTION,2014,148(4):343-352.
APA	Chang, Wen-Lin.,Yang, Qing.,Zhang, Hui.,Lin, Hai-Yan.,Zhou, Zhi.,...&Wang, Hongmei.(2014).Role of placenta-specific protein 1 in trophoblast invasion and migration.REPRODUCTION,148(4),343-352.
MLA	Chang, Wen-Lin,et al."Role of placenta-specific protein 1 in trophoblast invasion and migration".REPRODUCTION 148.4(2014):343-352.