Ultra-long silver nanowires induced mitotic abnormalities and cytokinetic failure in A549 cells

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境纳米材料实验室

	Ultra-long silver nanowires induced mitotic abnormalities and cytokinetic failure in A549 cells
	Wang, Fengbang ; Chen, Ying ; Wang, Yuanyuan ; Yin, Yongguang ; Qu, Guangbo ; Song, Maoyong ; Wang, Hailin
刊名	NANOTOXICOLOGY
	2019-04-21
卷号	13 期号:4 页码:543-557
关键词	Ultra-long silver nanowires mitotic abnormalities cytokinetic failure multinucleation ROS
ISSN号	1743-5390
英文摘要	Asbestos fiber has been associated with mesothelioma and lung cancer. However, the carcinogenic risks of other fiber nanomaterials with morphological similarities to asbestos have not been fully studied. Ultra-long silver nanowires (AgNWs) are increasingly used fiber-shaped nanomaterials with a high aspect ratio, but very few studies have investigated their health risks. Here, proliferation abnormalities of lung epithelial cells induced by ultra-long AgNWs were investigated. Ultra-long AgNW treatment induced dose- and diameter-dependent increase in the ratio of multinucleated cells. Further, proteins involved in mitosis and cytokinesis, including Aurora A, p-Histone 3 (ser10), RhoA, p-MLC, and myosin IIb, were significantly upregulated after an ultra-long AgNW treatment, leading to mitotic abnormalities and cytokinetic failure. Meanwhile, exposure to ultra-long AgNWs induced cell cycle arrest. Interestingly, a series of experiments demonstrated that ROS generation and Ag+ release were not responsible for the multinucleation induced by ultra-long AgNWs, but ultra-long AgNWs in the intercellular bridge might obstruct the contractile ring and inhibit abscission of the cytokinetic furrow by direct physical contact. Altogether, our findings indicate that ultra-long AgNWs can induce chromosomal instability, which has important consequences for the safety of ultra-long AgNWs to human health.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43217]
专题	生态环境研究中心_环境纳米材料实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Environm Nanotechnol & Hlth Effects, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Fengbang,Chen, Ying,Wang, Yuanyuan,et al. Ultra-long silver nanowires induced mitotic abnormalities and cytokinetic failure in A549 cells[J]. NANOTOXICOLOGY,2019,13(4):543-557.
APA	Wang, Fengbang.,Chen, Ying.,Wang, Yuanyuan.,Yin, Yongguang.,Qu, Guangbo.,...&Wang, Hailin.(2019).Ultra-long silver nanowires induced mitotic abnormalities and cytokinetic failure in A549 cells.NANOTOXICOLOGY,13(4),543-557.
MLA	Wang, Fengbang,et al."Ultra-long silver nanowires induced mitotic abnormalities and cytokinetic failure in A549 cells".NANOTOXICOLOGY 13.4(2019):543-557.