An Integrative Investigation ofParabistichella variabilis(Protista, Ciliophora, Hypotrichia) Including Its General Morphology, Ultrastructure, Ontogenesis, and Molecular Phylogeny

doi:10.1111/jeu.12809

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生物分类与系统演化实验室

	An Integrative Investigation ofParabistichella variabilis(Protista, Ciliophora, Hypotrichia) Including Its General Morphology, Ultrastructure, Ontogenesis, and Molecular Phylogeny
	Dong, Jingyi 1,5,6; Chen, Xumiao 4; Liu, Yongqiang 1,6; Ni, Bing 5; Fan, Xinpeng 5; Li, Lifang 2; Warren, Alan 3
刊名	JOURNAL OF EUKARYOTIC MICROBIOLOGY
	2020-06-15
页码	17
关键词	Buccal seal contractile vacuole pore extrusomes pharyngeal disks taxonomic assignment
ISSN号	1066-5234
DOI	10.1111/jeu.12809
通讯作者	Fan, Xinpeng(xpfan@bio.ecnu.edu.cn) ; Li, Lifang(qd_liliy@sina.com)
英文摘要	Hypotrichs are a highly differentiated and very diverse group of ciliated protists. Their systematics and taxonomy are challenging and call for detailed investigations on their general morphology, ultrastructure, ontogenesis, and molecular phylogeny. Here, a comprehensive study is conducted on a brackish water population ofParabistichella variabilisusing light and electron microscopy and phylogenetic analyses based on small subunit ribosomalDNAsequence data. Its morphology, including the infraciliature, pellicle, nuclei, buccal seal, and extrusomes, is documented. The present findings indicate that inP. variabilis: (i) the cortical granules are extrusomes, which differ from those of other hypotrichs; (ii) the buccal seal is bounded by the plasma membrane and contains a single layer of longitudinal microtubules; (iii) two contractile vacuoles might be present rather than one; and (iv) the pharyngeal disks are bounded by a single membrane. Early-to-middle stages of ontogenesis are described for the first time, enabling the complete characterization of this process. Phylogenetic analyses indicate thatParabistichella variabilisis closely related to several species from different genera, such asOrthoamphisiella breviseries,Uroleptoides magnigranulosus, andTachysoma pellionellum. However, ultrastructural and gene sequence data for more taxa are needed in order to resolve the systematics ofParabistichella.
资助项目	National Nature Science Foundation of China[41876151] ; National Nature Science Foundation of China[31772431] ; National Nature Science Foundation of China[31672249] ; Nature Science Foundation of Shandong Province[ZR2019QD015]
WOS研究方向	Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000540698500001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167723]
专题	海洋研究所_海洋生物分类与系统演化实验室
通讯作者	Fan, Xinpeng; Li, Lifang
作者单位	1.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China 2.Shandong Univ, Marine Coll, Weihai 264209, Peoples R China 3.Nat Hist Museum, Dept Life Sci, London SW7 5BD, England 4.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China 5.East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China 6.Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Dong, Jingyi,Chen, Xumiao,Liu, Yongqiang,et al. An Integrative Investigation ofParabistichella variabilis(Protista, Ciliophora, Hypotrichia) Including Its General Morphology, Ultrastructure, Ontogenesis, and Molecular Phylogeny[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2020:17.
APA	Dong, Jingyi.,Chen, Xumiao.,Liu, Yongqiang.,Ni, Bing.,Fan, Xinpeng.,...&Warren, Alan.(2020).An Integrative Investigation ofParabistichella variabilis(Protista, Ciliophora, Hypotrichia) Including Its General Morphology, Ultrastructure, Ontogenesis, and Molecular Phylogeny.JOURNAL OF EUKARYOTIC MICROBIOLOGY,17.
MLA	Dong, Jingyi,et al."An Integrative Investigation ofParabistichella variabilis(Protista, Ciliophora, Hypotrichia) Including Its General Morphology, Ultrastructure, Ontogenesis, and Molecular Phylogeny".JOURNAL OF EUKARYOTIC MICROBIOLOGY (2020):17.