Genome-wide association analyses based on whole-genome sequencing of Protosalanx hyalocranius provide insights into sex determination of Salangid fishes

doi:10.1111/1755-0998.13172

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	Genome-wide association analyses based on whole-genome sequencing of Protosalanx hyalocranius provide insights into sex determination of Salangid fishes
	Li, Yu-Long 1,2,3; Xing, Teng-Fei 1,2,3,4; Liu, Jin-Xian 1,2,3
刊名	MOLECULAR ECOLOGY RESOURCES
	2020-05-22
页码	12
关键词	genome-wide association analyses Salangid fish sex determination sex differentiation
ISSN号	1755-098X
DOI	10.1111/1755-0998.13172
通讯作者	Liu, Jin-Xian(jinxianliu@gmail.com)
英文摘要	Identification of sex determination system and sex-determining genes have important implications in conservation, ecology and evolution. However, much remains to be discovered about the evolution of different sexual determination systems in teleost fishes, of which the mechanisms of sex determination are remarkably variable. In the present study, the whole genomes of 20 males and 20 females of a Salangid fish, Protosalanx hyalocranius, were sequenced and genome wide association analyses were conducted to uncover its sex determination system and putative sex-determining genes. A total of 150 SNPs were significantly associated with sex, which showed high differentiation between sexes (F-ST ranged from 0.245 to 0.556). Of the 150 sex-associated SNPs, 76 SNPs displayed sex specificity with even coverage of depth and were female heterogametic, which suggested a ZZ/ZW sex determination system. Interestingly, one scaffold containing sex-specific SNPs displayed synteny to the sex chromosome of medaka. Annotations of sex-associated loci suggested that both transcriptional regulators (e.g., FOX genes) and secreted hormones and their receptors might be involved in the sex determination/differentiation of P. hyalocranius. More strikingly, we found a nonsense mutation in one copy of GALNT homology gene of all females, which suggested that "Z dosage" effect might play a vital role in the processes of sex determination/differentiation. These sex-specific loci could be a valuable resource for further research on sex determination of Salangid fishes and the results could contribute to the understanding of sex determination mechanisms and the evolution of sex chromosome in teleost fishes.
资助项目	National Natural Science Foundation of China[41676137] ; National Natural Science Foundation of China[41906080] ; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao)[LMEES-YTSP-2018-01-05]
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
语种	英语
出版者	WILEY
WOS记录号	WOS:000534579200001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167530]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Liu, Jin-Xian
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Yu-Long,Xing, Teng-Fei,Liu, Jin-Xian. Genome-wide association analyses based on whole-genome sequencing of Protosalanx hyalocranius provide insights into sex determination of Salangid fishes[J]. MOLECULAR ECOLOGY RESOURCES,2020:12.
APA	Li, Yu-Long,Xing, Teng-Fei,&Liu, Jin-Xian.(2020).Genome-wide association analyses based on whole-genome sequencing of Protosalanx hyalocranius provide insights into sex determination of Salangid fishes.MOLECULAR ECOLOGY RESOURCES,12.
MLA	Li, Yu-Long,et al."Genome-wide association analyses based on whole-genome sequencing of Protosalanx hyalocranius provide insights into sex determination of Salangid fishes".MOLECULAR ECOLOGY RESOURCES (2020):12.