Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure

doi:10.3389/fmars.2020.603825

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure
	Chen, Hao 2,3; Xin, Lusheng 3; Wang, Lin 3; Zhang, Huan 2,3; Liu, Rui 3; Wang, Hao 2,3; Qiao, Xue 1,4; Wang, Lingling 1,3; Song, Linsheng 1,4
刊名	FRONTIERS IN MARINE SCIENCE
	2021-01-11
卷号	7 页码:11
关键词	intertidal zone calmodulin nitrite oxide tumor necrosis factor aerial exposure
DOI	10.3389/fmars.2020.603825
通讯作者	Zhang, Huan(zhanghuan@qdio.ac.cn) ; Wang, Lingling(wanglingling@dlou.edu.cn)
英文摘要	Aerial exposure (emersion) due to the periodical ebb and flow of tides is a major stressor for intertidal organisms and a key environmental factor in shaping their local communities. Oysters are among the most emersion-tolerant mollusk species and can survive for several days under aerial exposure. Noticeably, overwhelming inflammation responses could occur during the emersion stress. However, mechanisms beneath the activation and modulation of emersion-induced inflammation response have remained largely unknown. Ca2+ is an important intracellular second messenger that plays indispensable roles in inflammation response by cooperation with calmodulin (CaM) genes. Here, we showed that intracellular Ca2+ accumulates rapidly in oyster hemocytes during emersion stress along with the changes in the protein levels of three CaM genes, which function as intracellular sensors of Ca2+. As downstream effector of Ca2+/CaM complex, nitric oxide synthase (NOS) activity in hemocytes was enhanced during the emersion stress, facilitating a greater production of nitrite oxide (NO). Augmentation of NO concentration was associated with the increased mRNA expression levels of two oyster cytokines (CgTNFs) during aerial exposure. The robust accumulation of cytokines and severe injury of tissues in oysters have been regarded as potential cause and marker of their death in prolonged emersion stress. Here, both the expression levels of CgTNFs and the tissue injuries of oysters were attenuated when Ca2+/CaM complex or NOS activity were repressed in vivo during the emersion stress. These findings indicate that Ca2+/CaM-NOS/NO-CgTNFs pathway is critically involved in the emersion-induced inflammation response in oysters and plays a role in the resistance against long-term aerial exposure.
资助项目	National Key R&D Program from the National Science Foundation of China[2018YFD0900606] ; National Key R&D Program from the National Science Foundation of China[41961124009] ; National Key R&D Program from the National Science Foundation of China[U1706204] ; Modern Agro-industry Technology Research System[CARS-49] ; Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research, Key R&D Program of Liaoning Province[2017203001] ; Aoshan Talents Cultivation Program - Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Distinguished Professor of Liaoning ; Research Foundation for Talented Scholars in Dalian Ocean University
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000611158900001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/170090]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Zhang, Huan; Wang, Lingling
作者单位	1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Chen, Hao,Xin, Lusheng,Wang, Lin,et al. Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure[J]. FRONTIERS IN MARINE SCIENCE,2021,7:11.
APA	Chen, Hao.,Xin, Lusheng.,Wang, Lin.,Zhang, Huan.,Liu, Rui.,...&Song, Linsheng.(2021).Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure.FRONTIERS IN MARINE SCIENCE,7,11.
MLA	Chen, Hao,et al."Ca2+/Calmodulin-NOS/NO-TNFs Pathway Hallmarks the Inflammation Response of Oyster During Aerial Exposure".FRONTIERS IN MARINE SCIENCE 7(2021):11.