Transcriptomic analysis and expression of C-type lectins in response to Vibrio parahaemolyticus challenge in Scapharca subcrenata

doi:10.1016/j.fsi.2020.08.016

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Transcriptomic analysis and expression of C-type lectins in response to Vibrio parahaemolyticus challenge in Scapharca subcrenata
	Lin, Jiliang 1; Ning, Junhao 2; Lu, Xia 2; Chen, Min 2; Cao, Weian 1; Wang, Chunde 1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2020-11-01
卷号	106 页码:365-373
关键词	Scapharca subcrenata Transcriptome Maternal immunity C-type lectin Expression analysis
ISSN号	1050-4648
DOI	10.1016/j.fsi.2020.08.016
通讯作者	Wang, Chunde(chundewang2007@163.com)
英文摘要	Little information is available on innate immune defense mechanisms of Scapharca subcrenata. C-type lectins (CTLs) are not only pattern recognition proteins that can bind pathogen-associated molecular patterns, but also crucial maternally-derived immune factors in mollusc egg. In this study, the comparative transcriptome analysis of Vibrio parahaemolyticus-infected and untreated hepatopancreas were performed to identify the key genes involved in maternal transfer of immunity. A total of 3514 and 9327 differentially expressed genes (DEGs) were identified at 6 and 48 h post challenge compared to control groups. Gene Ontology and Cluster of Orthologous Groups analysis showed that most DEGs were classified under regulation of signal transduction, regulation of the metabolic process of carbohydrates and secondary metabolites, while the processes of posttranscriptional modification and protein translation were inhibited manifestly. The DEGs were most enriched in pathways related to lysosome, phagosome and EMC-receptor interaction. Among the DEGs, 191 maternal immune-related genes that could provide developing embryos a better protection against pathogen infection were identified according to previous studies. Additionally, five CTLs (designated as SsCTL1-5) identified from the DEGs were cloned, and their expression patterns in different tissues and post immune stimulation were analyzed. These findings would be beneficial for understanding the innate immune defense mechanisms of S. subcrenata.
资助项目	National Key R&D Program of China[2019YFD0900704] ; National Natural Science Foundation of China[31972791] ; Scientific and Technological Project of Yantai, Shandong Province, China[2019LJRC141] ; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences, China[ZR2017QD001]
WOS关键词	SCALLOP ARGOPECTEN-IRRADIANS ; MATERNAL TRANSFER ; CHLAMYS-FARRERI ; FUNCTIONAL-CHARACTERIZATION ; BINDING ; EGG ; IDENTIFICATION ; RECOGNITION ; COMPLEMENT ; COMPONENTS
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
WOS记录号	WOS:000579494400036
资助机构	National Key R&D Program of China ; National Natural Science Foundation of China ; Scientific and Technological Project of Yantai, Shandong Province, China ; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences, China
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/28458]
专题	海岸带生物学与生物资源利用重点实验室中国科学院烟台海岸带研究所海岸带生物资源高效利用研究与发展中心
通讯作者	Wang, Chunde
作者单位	1.Qingdao Agr Univ, Qingdao 266109, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Lin, Jiliang,Ning, Junhao,Lu, Xia,et al. Transcriptomic analysis and expression of C-type lectins in response to Vibrio parahaemolyticus challenge in Scapharca subcrenata[J]. FISH & SHELLFISH IMMUNOLOGY,2020,106:365-373.
APA	Lin, Jiliang,Ning, Junhao,Lu, Xia,Chen, Min,Cao, Weian,&Wang, Chunde.(2020).Transcriptomic analysis and expression of C-type lectins in response to Vibrio parahaemolyticus challenge in Scapharca subcrenata.FISH & SHELLFISH IMMUNOLOGY,106,365-373.
MLA	Lin, Jiliang,et al."Transcriptomic analysis and expression of C-type lectins in response to Vibrio parahaemolyticus challenge in Scapharca subcrenata".FISH & SHELLFISH IMMUNOLOGY 106(2020):365-373.