Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli

doi:10.1007/s10811-021-02408-0

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli
	Li, Wenjun 1,2; Ma, Chengbo 1,3; Ge, Baosheng 4; Zang, Fan 1,3; Qin, Song 1,2; Lin, Jian 3
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2021-04-29
页码	11
关键词	Combinational biosynthesis Phycoerythrobilin Response surface methodology Extraction
ISSN号	0921-8971
DOI	10.1007/s10811-021-02408-0
通讯作者	Qin, Song(sqin@yic.ac.cn) ; Lin, Jian(linjian3384@163.com)
英文摘要	Phycoerythrobilin (PEB) is a special pigment in red algae and cyanobacteria that plays an important role in capturing light energy. As a natural non-toxic pigment with antioxidant properties, PEB is widely used in food and cosmetics industries. At present, the presence of other pigments in red algae and cyanobacteria, such as chlorophyll and phycocyanobilin, makes the preparation of PEB complicated and costly. In this study, the PEB synthesizing enzyme genes of hox1 from Synechocystis sp. PCC6803 and pebS from Nostoc sp. PCC 7120 were co-expressed in Escherichia coli to produce recombinant PEB. In addition, single-factor and response surface methods were used to enhance the yield of recombinant PEB. Under the conditions of lactose concentration at 4 mmol L-1, induction temperature at 27 degrees C, OD600 nm of 0.9, and induction time at 14 h, the concentration of PEB reached 20.37 mg L-1. In addition, a high-purity (purity > 70%) PEB was obtained through chloroform extraction, and the purified product was verified in UV spectrometry, high-performance liquid chromatography, and mass spectrometry.
资助项目	National Natural Science Foundation of China[41906109] ; National Natural Science Foundation of China[42061134020] ; National Natural Science Foundation of China[41411130202] ; Key Research and Development Program of Yantai City, Shandong, China[2019XDHZ101] ; Key Research and Development Program of Yantai City, Shandong, China[2020MSGY084]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000645498300002
资助机构	National Natural Science Foundation of China ; Key Research and Development Program of Yantai City, Shandong, China
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/27223]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Qin, Song; Lin, Jian
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China
推荐引用方式 GB/T 7714	Li, Wenjun,Ma, Chengbo,Ge, Baosheng,et al. Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli[J]. JOURNAL OF APPLIED PHYCOLOGY,2021:11.
APA	Li, Wenjun,Ma, Chengbo,Ge, Baosheng,Zang, Fan,Qin, Song,&Lin, Jian.(2021).Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli.JOURNAL OF APPLIED PHYCOLOGY,11.
MLA	Li, Wenjun,et al."Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli".JOURNAL OF APPLIED PHYCOLOGY (2021):11.