Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli

doi:10.1016/j.procbio.2018.05.011

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli
	Ge, Baosheng ; Chen, Yao ; Yu, Qian ; Lin, Xiaojun ; Li, Jiqiang ; Qin, Song
刊名	PROCESS BIOCHEMISTRY
	2018-08
卷号	71 页码:23-30
关键词	Phycocyanobilin Heme biosynthetic pathway 5-Aminolevulinic acid Regulation Combinatorial biosynthesis Escherichia coli
ISSN号	1359-5113
DOI	10.1016/j.procbio.2018.05.011
产权排序	[Ge, Baosheng ; Chen, Yao ; Yu, Qian ; Lin, Xiaojun ; Li, Jiqiang] China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China ; [Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone, Yantai 264003, Peoples R China
文献子类	Article
英文摘要	Due to its biochemical and spectroscopic properties, phycocyanobilin (PCB) is of special interest for a variety of applications, but the production of PCB from Spirulina is complicated and costly. Here we report the assembly and regulation of the entire heme biosynthetic pathway for combinatorial biosynthesis of PCB in Escherichia coli. Our results shows that a plasmid containing bicistronic expression of hoxi and pcyA yields 6.64 mg/L PCB, which is higher than that of plasmid with one promoter for both genes (p < 0.01). Up-regulation of hemB, hemG, hemH, hemA(s), and hemL individually or simultaneously does not significantly improve the yield of PCB, but induces accumulation of 5-Aminolevulinic acid (ALA) and heme. Increasing the copy number of hoxi and pcyA genes slightly improves the production of PCB by enhancing the expression level of Hox1 and PcyA enzymes, suggesting that this two-step enzymatic reaction is the rate-limiting step for the production of PCB. This work provides new important insights into the regulatory mechanism of the heme biosynthesis pathway for production of valuable chemicals.
WOS关键词	5-AMINOLEVULINIC ACID ; BILE-PIGMENTS ; ALLOPHYCOCYANIN ; TETRAPYRROLES ; CHROMOPHORE ; DISORDERS ; SPIRULINA ; EFFICIENT ; GLUCOSE ; PROTEIN
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
语种	英语
WOS记录号	WOS:000442004200004
资助机构	National key Research and Development Plan of China [2016YFE0106700] ; National Natural Science Foundation of China [21373271, 21673294] ; Key Research Project of Shandong Province [2017GGX40114] ; Open Fund of Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China [OF2015N009] ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24506]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Ge, Baosheng,Chen, Yao,Yu, Qian,et al. Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli[J]. PROCESS BIOCHEMISTRY,2018,71:23-30.
APA	Ge, Baosheng,Chen, Yao,Yu, Qian,Lin, Xiaojun,Li, Jiqiang,&Qin, Song.(2018).Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli.PROCESS BIOCHEMISTRY,71,23-30.
MLA	Ge, Baosheng,et al."Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli".PROCESS BIOCHEMISTRY 71(2018):23-30.