热刺激对栉孔扇贝免疫功能和热休克蛋白表达的影响

doi:DOI:10.16441/j.cnki.hdxb.20160366

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中国科学院牟平海岸带环境综合试验站

	热刺激对栉孔扇贝免疫功能和热休克蛋白表达的影响
	刘甜雨 ; 王清 ; 陈慕雁
刊名	中国海洋大学学报. 自然科学版
	2017-08-15
卷号	47 期号:8 页码:31-43
关键词	HSP70 HSP90 thermal stimulus Chlamysfarreri hemocyte immune response apoptosis DNA damage HSP70 HSP90 热刺激栉孔扇贝血细胞免疫应答细胞凋亡 DNA损伤
ISSN号	1672-5174
DOI	DOI:10.16441/j.cnki.hdxb.20160366
其他题名	Effect of Thermal Stimulus on Immune Function and Heat Shock Protein Expression of Scallop Chlamys farreri
产权排序	(1) 海水养殖教育部重点实验室(中国海洋大学) ; (2) 中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室 ; (3) 中国科学院烟台海岸带研究所牟平海岸带环境综合试验站
文献子类	Article
英文摘要	The objective of this study was to evaluate the effect of thermal stimulus on the scallop, Chlamysfarreri,which was generally recognized as a temperature sensitive bivalve species.Immune response, DNA damage,apoptosis and transcription of heat shock protein(HSP70and HSP90)genes in hemocytes were evaluated over a short period of thermal stimulus.The hemocyte of scallops was obtained on 0,1,2,4,8hafter being treated at 28℃.By using flow cytometry,reactive oxygen species (ROS)production and phagocytosis activity of hemocytes were estimated.The results demonstrated that both ROS and phagocytosis activity reached the highest level in 4hand reduced markedly by 8h. The stress also significantly decreased antibacterial and bacteriolytic activities.By contrast,the DNA damage(mainly DNA breakages)level in hemocytes as was detected by the alkaline comet assay increased following high-temperature exposure,and hemocyte apoptosis was observed under fluorescence microscope.Significant increases in the transcription of HSP70and HSP90genes were also detected in hemocytes,while the transcription of HSP70grew faster than HSP90did and reached to a higher level. This study demonstrated that immune response,DNA damage level and apoptosis of hemocytes in the scallop were strongly affected within several hours,and the transcripts of HSPs genes were strengthened so as to protect the cells and tissues from injury when C.farreri subjected to short-term high temperature stressing.
WOS关键词	Fisheries
WOS研究方向	Fisheries ; 渔业
语种	中文
CSCD记录号	CSCD:6023284
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24292]
专题	中国科学院牟平海岸带环境综合试验站海岸带生物资源高效利用研究与发展中心
作者单位	1.海水养殖教育部重点实验室(中国海洋大学); 2.中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室; 3.中国科学院烟台海岸带研究所牟平海岸带环境综合试验站
推荐引用方式 GB/T 7714	刘甜雨,王清,陈慕雁. 热刺激对栉孔扇贝免疫功能和热休克蛋白表达的影响[J]. 中国海洋大学学报. 自然科学版,2017,47(8):31-43.
APA	刘甜雨,王清,&陈慕雁.(2017).热刺激对栉孔扇贝免疫功能和热休克蛋白表达的影响.中国海洋大学学报. 自然科学版,47(8),31-43.
MLA	刘甜雨,et al."热刺激对栉孔扇贝免疫功能和热休克蛋白表达的影响".中国海洋大学学报. 自然科学版 47.8(2017):31-43.