A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity

doi:10.1039/d0an00060d

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity
	Wen, Jiahui ; Liu, Yongming ; Li, Jingwen ; Lin, Hao ; Zheng, Yiran ; Chen, Yan ; Fu, Xiuli ; Chen, Lingxin
刊名	ANALYST
	2020-04-07
卷号	145 期号:7 页码:2774-2778
关键词	SINGLE-STRANDED-DNA TURN-ON DETECTION COPPER NANOPARTICLES ENZYMATIC CLEAVAGE OXIDATIVE DAMAGE ASSAY FLUORESCENCE INHIBITION GOLD UNIVERSAL
DOI	10.1039/d0an00060d
产权排序	[Wen, Jiahui ; Liu, Yongming ; Li, Jingwen ; Lin, Hao ; Zheng, Yiran ; Fu, Xiuli] Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China ; [Chen, Yan] Shandong Agr & Engn Univ, Sch Resources & Environm Engn, Jinan 250100, Peoples R China ; [Chen, Lingxin] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China ; [Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
文献子类	Article
英文摘要	A label-free, sensitive, simple and general colorimetric method was reported to monitor S1 nuclease activity based on protamine-assisted aggregation of gold nanoparticles (AuNPs). Here, protamine, a linear polycation, was used as a medium for causing the aggregation of negatively charged AuNPs by electrostatic interactions, resulting in changes in the surface plasmon resonance (SPR) absorption bands as well as the color of AuNPs. Here, the AuNPs were employed as an indicator to detect the level of S1 nuclease in the solution. Substrate DNA could be cleaved into small fragments by the specific S1 nuclease, which effectively prevents the electrostatic interaction between DNA and protamine and thus facilitates the interaction between protamine and AuNPs. The quantitative analysis of S1 nuclease activity can be performed via directly measuring the changes in the absorption spectra of the AuNPs. Using S1 nuclease as a model analyte, the limit of detection was estimated to be 1.0 x 10(-4) U mL(-1). Furthermore, the proposed concept has been successfully applied in S1 nuclease analysis of serum samples, offering an ultrasensitive strategy for the speedy detection of the nuclease activity and providing a new avenue for high-throughput screening of nucleases and drugs with potential inhibition properties.
WOS关键词	SINGLE-STRANDED-DNA ; TURN-ON DETECTION ; COPPER NANOPARTICLES ; ENZYMATIC CLEAVAGE ; OXIDATIVE DAMAGE ; ASSAY ; FLUORESCENCE ; INHIBITION ; GOLD ; UNIVERSAL
语种	英语
WOS记录号	WOS:000526825700033
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21705139, 61801274, 21976209] ; Natural Science Foundation of Shandong Province in ChinaNatural Science Foundation of Shandong Province [ZR2017BB026] ; Taishan Scholar Project Special Funding [ts20190962]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/25053]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Shandong Agr & Engn Univ, Sch Resources & Environm Engn, Jinan 250100, Peoples R China; 2.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China; 3.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Wen, Jiahui,Liu, Yongming,Li, Jingwen,et al. A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity[J]. ANALYST,2020,145(7):2774-2778.
APA	Wen, Jiahui.,Liu, Yongming.,Li, Jingwen.,Lin, Hao.,Zheng, Yiran.,...&Chen, Lingxin.(2020).A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity.ANALYST,145(7),2774-2778.
MLA	Wen, Jiahui,et al."A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity".ANALYST 145.7(2020):2774-2778.