Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay

doi:10.1021/acs.analchem.8b05095

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay
	Chen, Zhaopeng ; Wang, Han ; Zhang, Zhiyang ; Chen, Lingxin
刊名	ANALYTICAL CHEMISTRY
	2019-01-15
卷号	91 期号:2 页码:1254-1259
关键词	ALKALINE-PHOSPHATASE ACTIVITY SIGNAL AMPLIFICATION PLASMONIC ELISA ORGANIC FRAMEWORKS PEROXIDASE-LIKE IMMUNOASSAY ACID HYDROQUINONE SUBSTRATE DESIGN
ISSN号	0003-2700
DOI	10.1021/acs.analchem.8b05095
产权排序	[Chen, Zhaopeng ; Wang, Han ; Zhang, Zhiyang ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China ; [Chen, Zhaopeng ; Wang, Han ; Zhang, Zhiyang ; Chen, Lingxin] YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China ; [Chen, Zhaopeng] Xian Univ Technol, Sch Sci, Dept Appl Chem, Xian 710061, Shaanxi, Peoples R China ; [Chen, Lingxin] Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China ; [Wang, Han ; Zhang, Zhiyang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
文献子类	Article
英文摘要	Herein, a redox-cycling was proposed to amplify the signal of enzyme-linked immunosorbent assay (ELISA), which was performed in a polystyrene microplate based on a classic sandwich-type. After the sandwich immunoreactions were finished, the alkaline phosphatase captured on a microplate triggered the hydrolyzation of i-ascorbic acid 2-phosphate to generate ascorbic acid (AA), which then reduced colorless tris(bathophenanthroline) iron(III) (Fe(BPT)(3)(3+)) encapsulated in the micelle of TX-100 to pink red tris(bathophenanthroline) iron(II) (Fe(BPT)(3)(2+). In the presence of tris(2-carboxyethyl)phosphine, the oxidation product, dehydroascorbic acid, was transformed to AA quickly which then reduced Fe(BPT)(3)(3+) again and again, resulting in the generation of abundant Fe(BPT)(3)(2+) that could be read out conveniently by a commercial microplate reader or the naked eye. Because the negative charged TCEP with large size could hardly pass through the micelle, the reduction of Fe(BPT)(3)(3+) by TCEP directly was negligible. Experiment results for assay of alpha-fetoprotein (a model antigen) showed the cycling greatly improved the detection limit to 5 pg/mL, 2 orders of magnitude lower than that of conventional ELISA. The cycling also exhibited the advantages of simplicity and high reproducibility, implying its great potential for practical applications in biological and clinical diagnosis.
WOS关键词	ALKALINE-PHOSPHATASE ACTIVITY ; SIGNAL AMPLIFICATION ; PLASMONIC ELISA ; ORGANIC FRAMEWORKS ; PEROXIDASE-LIKE ; IMMUNOASSAY ; ACID ; HYDROQUINONE ; SUBSTRATE ; DESIGN
WOS研究方向	Chemistry, Analytical
语种	英语
WOS记录号	WOS:000456350000009
资助机构	National Natural Science Foundation of China [21575159, 21804010, 41601525, 41776110] ; Natural Science Foundation of Shandong Province of China [ZR2014BL031, ZR2016DB07, ZR2016BL25]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24625]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China; 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Xian Univ Technol, Sch Sci, Dept Appl Chem, Xian 710061, Shaanxi, Peoples R China; 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China;
推荐引用方式 GB/T 7714	Chen, Zhaopeng,Wang, Han,Zhang, Zhiyang,et al. Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay[J]. ANALYTICAL CHEMISTRY,2019,91(2):1254-1259.
APA	Chen, Zhaopeng,Wang, Han,Zhang, Zhiyang,&Chen, Lingxin.(2019).Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay.ANALYTICAL CHEMISTRY,91(2),1254-1259.
MLA	Chen, Zhaopeng,et al."Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay".ANALYTICAL CHEMISTRY 91.2(2019):1254-1259.