Extended field of view of light-sheet fluorescence microscopy by scanning multiple focus-shifted Gaussian beam arrays | |
Liu, Chao1,2; Bai, Chen2; Yu, Xianghua2; Yan, Shaohui2; Zhou, Yuan1,2; Li, Xing1,2; Min, Junwei2; Yang, Yanlong2; Dan, Dan2; Yao, Baoli1,2 | |
刊名 | Optics Express |
2021-02-15 | |
卷号 | 29期号:4页码:6158-6168 |
ISSN号 | 10944087 |
DOI | 10.1364/OE.418707 |
产权排序 | 1 |
英文摘要 | Light-sheet fluorescence microscopy (LSFM) facilitates high temporal-spatial resolution, low photobleaching and phototoxicity for long-term volumetric imaging. However, when a high axial resolution or optical sectioning capability is required, the field of view (FOV) is limited. Here, we propose to generate a large FOV of light-sheet by scanning multiple focus-shifted Gaussian beam arrays (MGBA) while keeping the high axial resolution. The positions of the beam waists of the multiple Gaussian beam arrays are shifted in both axial and lateral directions in an optimized arranged pattern, and then scanned along the direction perpendicular to the propagation axis to form an extended FOV of light-sheet. Complementary beam subtraction method is also adopted to further improve axial resolution. Compared with the single Gaussian light-sheet method, the proposed method extends the FOV from 12 µm to 200 µm while sustaining the axial resolution of 0.73 µm. Both numerical simulation and experiment on samples are performed to verify the effectiveness of the method. © 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
语种 | 英语 |
出版者 | OSA - The Optical Society |
WOS记录号 | WOS:000619209800120 |
内容类型 | 期刊论文 |
源URL | [http://ir.opt.ac.cn/handle/181661/94442] |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
作者单位 | 1.University of Chinese Academy of Sciences, Beijing; 100049, China 2.State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and PrecisionMechanics, Chinese Academy of Sciences, Xi’an, Shaanxi; 710119, China; |
推荐引用方式 GB/T 7714 | Liu, Chao,Bai, Chen,Yu, Xianghua,et al. Extended field of view of light-sheet fluorescence microscopy by scanning multiple focus-shifted Gaussian beam arrays[J]. Optics Express,2021,29(4):6158-6168. |
APA | Liu, Chao.,Bai, Chen.,Yu, Xianghua.,Yan, Shaohui.,Zhou, Yuan.,...&Yao, Baoli.(2021).Extended field of view of light-sheet fluorescence microscopy by scanning multiple focus-shifted Gaussian beam arrays.Optics Express,29(4),6158-6168. |
MLA | Liu, Chao,et al."Extended field of view of light-sheet fluorescence microscopy by scanning multiple focus-shifted Gaussian beam arrays".Optics Express 29.4(2021):6158-6168. |
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