Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus

doi:10.1016/j.indcrop.2021.113448

	Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus
	Zhong, Zhuoheng 1,2; Liu, Shengzhi 2; Han, Songling 2; Li, Yaohan 2; Tao, Minglei 2; Liu, Amin 2; He, Qiang 3; Chen, Sixue 4; Dufresne, Criag 5; Zhu, Wei 1,6
刊名	INDUSTRIAL CROPS AND PRODUCTS
	2021-08-01
卷号	166
关键词	Mitochondrial respiratory complex Catharanthus roseus Ultraviolet B Alkaloids
ISSN号	0926-6690
DOI	10.1016/j.indcrop.2021.113448
通讯作者	Zhu, Wei(zhuwei2007100@163.com) ; Tian, Jingkui(tjk@zju.edu.cn)
英文摘要	Indole alkaloids from Catharanthus roseus (L.) G. Don were valued for their wide spectrum of pharmaceutical effects. Their biosynthesis is modulated by various abiotic factors including ultraviolet B radiation which induced ATP production and led to accumulation of indole alkaloids. To investigate the regulatory mechanism of secondary metabolism in the leaf mitochondria of C. roseus under ultraviolet B radiation, combinatory study of proteomic and metabolomic analyses of C. roseus was performed. ATP export in the leaves of C. roseus increased under UVB radiation, which required mitochondrial ATP synthase. Proteins related to mitochondrial complexes II/IV and their gene expression levels increased, and those related to mitochondrial complex I decreased. Proteins related to the methylerythritol phosphate pathway, especially geranylgeranyl pyrophosphate synthase, increased under ultraviolet B radiation. Metabolites whose levels changed were mainly alkaloids, organic acids, carbohydrates, phenylpropanoids, and fatty acids, while eight indole alkaloids increased. Integrative analysis of omics data indicated that the metabolism of glutamate and tyrosine was downregulated. These results suggest that ultraviolet B radiation induces dynamic changes in mitochondria in C. roseus, which guarantees ATP production, regulates the flux of the methylerythritol phosphate pathway to the biosynthesis of monoterpene moieties, and leads to accumulation of various indole alkaloids.
资助项目	National Natural Science Foundation of China[81872973] ; National Science and Technology Major project of China[2019ZX09301108] ; Jiangsu Provincial Natural Science Foundation of China[BK20190223]
WOS关键词	MITOCHONDRIAL ; BIOSYNTHESIS ; METABOLISM ; RESPIRATION ; PROTEOMICS ; STRESS ; ATP ; ORGANIZATION ; PROTEINS ; SYNTHASE
WOS研究方向	Agriculture
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000649652800003
资助机构	National Natural Science Foundation of China ; National Science and Technology Major project of China ; Jiangsu Provincial Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/122263]
专题	中国科学院合肥物质科学研究院
通讯作者	Zhu, Wei; Tian, Jingkui
作者单位	1.Univ Chinese Acad Sci, Canc Hosp, Zhejiang Canc Hosp, Inst Canc & Basic Med,Chinese Acad Sci, Hangzhou 310022, Peoples R China 2.Zhejiang Univ, Coll Biomed Engn & Instrument Sci, Hangzhou 310027, Peoples R China 3.Zhejiang Prov Peoples Hosp, Dept Nephrol, Hangzhou 310014, Peoples R China 4.Univ Florida, Interdisciplinary Ctr Biotechnol Res, Dept Biol Prote & Mass Spectrometry, Gainesville, FL 32610 USA 5.Thermo Fisher Sci, W Palm Beach, FL 33407 USA 6.Changshu Qiushi Technol Co Ltd, Suzhou 215500, Peoples R China
推荐引用方式 GB/T 7714	Zhong, Zhuoheng,Liu, Shengzhi,Han, Songling,et al. Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus[J]. INDUSTRIAL CROPS AND PRODUCTS,2021,166.
APA	Zhong, Zhuoheng.,Liu, Shengzhi.,Han, Songling.,Li, Yaohan.,Tao, Minglei.,...&Tian, Jingkui.(2021).Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus.INDUSTRIAL CROPS AND PRODUCTS,166.
MLA	Zhong, Zhuoheng,et al."Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus".INDUSTRIAL CROPS AND PRODUCTS 166(2021).