Application of 9.4T MRI in Wilson Disease Model TX Mice With Quantitative Susceptibility Mapping to Assess Copper Distribution

doi:10.3389/fnbeh.2020.00059

	Application of 9.4T MRI in Wilson Disease Model TX Mice With Quantitative Susceptibility Mapping to Assess Copper Distribution
	Han Yongsheng 2; Dong Jianjian 2; Xu Chenchen 2; Rao Rao 2; Shu Shan 2; Li Guangda 2; Cheng Nan 2; Wu Yun 1; Yang Hongyi 1; Han Yongzhu 2
刊名	FRONTIERS IN BEHAVIORAL NEUROSCIENCE
	2020-04-22
卷号	14
关键词	9 4-Tesla magnetic resonance penicillamine quantitative susceptibility mapping TX mouse Wilson disease
ISSN号	1662-5153
DOI	10.3389/fnbeh.2020.00059
通讯作者	Han Yongzhu(neurodoc@mail.ustc.edu.cn) ; Zhong Kai(kzhong@hmfl.ac.cn)
英文摘要	In the current study, we used 9.4-tesla magnetic resonance imaging (9.4T MRI) and inductively coupled plasma mass spectrometry (ICP-MS) to investigate the distribution of copper in the brain samples of a murine model of Wilson's disease (WD) following penicillamine (PCA) treatment. We also evaluated if the distribution of copper in the brain samples of mice was correlated with behavioral symptoms. Results from the behavioral experiments showed that 7 days of PCA treatment decreased the total distance traveled in the open field and the number of rearing and climbing instances among the toxic milk (TX) mice as compared with model group. We also observed that the open arm ratio in the elevated plus-maze (EPM) was reduced, escape latency in the Barnes maze test was increased, and avoidance in the open field was enhanced in TX mice following 14 days of PCA treatment as compared with those in untreated TX mice. We found that PCA treatment for 21-28 days improved the cognitive abilities, exploratory behavior, and movement behavior of TX mice. The PCA-treated mice also exhibited varying degrees of magnetic susceptibilities in the cortex, corpus striatum, hippocampus, and amygdaloid nucleus across the treatment period. Low copper concentrations were found in all of the analyzed brain regions of PCA-treated mice after 21-28 days as compared with the model group (P < 0.05). However, copper concentrations were increased in the primary motor cortex and cerebellum at 7 days post-PCA treatment as compared with those in the model group (P < 0.05). After 14 days of PCA treatment, the copper concentrations in the sensorimotor cortex, corpus striatum, hippocampus, and amygdaloid nucleus were higher than those detected without treatment. The results from a Pearson's correlation analysis revealed that there was a significant (P < 0.05) correlation between copper concentrations and magnetic susceptibility in all of the brain regions that were analyzed. Therefore, our results indicate that copper concentration and magnetic susceptibility are associated with alterations in mood-related behavior, recognition memory, and movement behaviors in TX mice that are treated with PCA. The redistribution of copper in the TX mouse brain during PCA treatment may aggravate changes in behavioral performance.
资助项目	National Natural Science Foundation of China[81774425] ; National Natural Science Foundation of China[81603596] ; National Natural Science Foundation of China[81573954] ; Natural Science Foundation of Anhui Province[1808085MH245]
WOS关键词	MOUSE
WOS研究方向	Behavioral Sciences ; Neurosciences & Neurology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000532381200001
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Anhui Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/103317]
专题	中国科学院合肥物质科学研究院
通讯作者	Han Yongzhu; Zhong Kai
作者单位	1.Chinese Acad Sci, High Magnet Field Lab, Hefei Inst Phys Sci, Hefei, Peoples R China 2.Anhui Univ TCM, Hosp Affiliated, Inst Neurol, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Han Yongsheng,Dong Jianjian,Xu Chenchen,et al. Application of 9.4T MRI in Wilson Disease Model TX Mice With Quantitative Susceptibility Mapping to Assess Copper Distribution[J]. FRONTIERS IN BEHAVIORAL NEUROSCIENCE,2020,14.
APA	Han Yongsheng.,Dong Jianjian.,Xu Chenchen.,Rao Rao.,Shu Shan.,...&Zhong Kai.(2020).Application of 9.4T MRI in Wilson Disease Model TX Mice With Quantitative Susceptibility Mapping to Assess Copper Distribution.FRONTIERS IN BEHAVIORAL NEUROSCIENCE,14.
MLA	Han Yongsheng,et al."Application of 9.4T MRI in Wilson Disease Model TX Mice With Quantitative Susceptibility Mapping to Assess Copper Distribution".FRONTIERS IN BEHAVIORAL NEUROSCIENCE 14(2020).