A water-soluble peptide fluorescent chemosensor for detection of cadmium (II) and copper (II) by two different response modes and its application in living LNcap cells

	A water-soluble peptide fluorescent chemosensor for detection of cadmium (II) and copper (II) by two different response modes and its application in living LNcap cells
	Wang, Peng; Wu, Jiang; Zhao, Chenhui
刊名	SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
	2020
卷号	226
关键词	Peptide Fluorescent chemosensor Cd2+ ions Cu2+ ions Cells imaging
英文摘要	A novel peptide-based fluorescent chemosensor (DGC) based on dansyl-appended dipeptide (Gly-Cys-NH2) was synthesized using SPPS technology. DGC exhibited highly sensitive detection of Cadmium (II) ions in 100% aqueous solutions through fluorescent "turn on" response and the detection limits of 14.5 nM. On the other hand, the fluorescence of DGC was almost completely quenched with fast response time when the addition of Cu2+ ions to DGC solutions, the detection limits for Cu2+ was 26.3 nM. In addition, the 2:1 binding stoichiometry of DGC with Cu2+ and Cu2+ were confirmed by Job's plot, fluorescent titration and HR-MS data. More importantly, MTT assays and fluorescence imaging experiments suggested that DGC has outstanding membrane permeability and hypotoxicity, and could be an efficient fluorescent chemosensor for Cu2+ and Cue - detection by two different response modes in living LNcap cells. (C) 2019 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60419]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Peng,Wu, Jiang,Zhao, Chenhui. A water-soluble peptide fluorescent chemosensor for detection of cadmium (II) and copper (II) by two different response modes and its application in living LNcap cells[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2020,226.
APA	Wang, Peng,Wu, Jiang,&Zhao, Chenhui.(2020).A water-soluble peptide fluorescent chemosensor for detection of cadmium (II) and copper (II) by two different response modes and its application in living LNcap cells.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,226.
MLA	Wang, Peng,et al."A water-soluble peptide fluorescent chemosensor for detection of cadmium (II) and copper (II) by two different response modes and its application in living LNcap cells".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 226(2020).