Optimization of the Fluorescent Protein Expression Level Based on Pseudorabies Virus Bartha Strain for Neural Circuit Tracing

doi:10.3389/fnana.2019.00063

	Optimization of the Fluorescent Protein Expression Level Based on Pseudorabies Virus Bartha Strain for Neural Circuit Tracing
	Jia, Fan 1,3,4; Lv, Pei 4; Miao, Huan 4; Shi, Xiangwei 1; Mei, Hongjun 5; Li, Li 4; Xu, Xiaoqin 4; Tao, Sijue 4; Xu, Fuqiang 1,2,3,4
刊名	FRONTIERS IN NEUROANATOMY
	2019-06-21
卷号	13 页码:9
关键词	PRV Bartha strain retrograde trans-multisynaptic tracer neural circuit PRV531 PRV724
ISSN号	1662-5129
DOI	10.3389/fnana.2019.00063
英文摘要	Mapping the neural circuits facilitates understanding the brain's working mechanism. Pseudorabies virus (PRV; Bartha stain) as a tracer can infect neurons and retrogradely transport in neural circuits. To illuminate the network, tracers expressing reporter genes at a high level are needed. In this study, we optimized the expression level of reporter genes and constructed two new retrograde trans-multisynaptic tracers PRV531 and PRV724, which separately express more robust green and red fluorescent proteins than the existing retrograde tracers PRV152 and PRV614. PRV531 and PRV724 can be used for mapping the neural circuit of the central nervous system (CNS) and the peripheral nervous system (PNS). Overall, our work adds two valuable tracers to the toolbox for mapping neural circuits.
资助项目	National Natural Science Foundation of China[31830035] ; National Natural Science Foundation of China[91732304] ; National Natural Science Foundation of China[31329001] ; National Basic Research Program (973 Program) of China[2015CB755600] ; Major Research Plan of the National Natural Science Foundation of China[91632303] ; [XDB32030200]
WOS关键词	POSTTRANSCRIPTIONAL REGULATORY ELEMENT ; GENE ; NEURONS ; VECTOR ; CONNECTIONS ; TRANSGENES ; CERVIX
WOS研究方向	Anatomy & Morphology ; Neurosciences & Neurology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000472658900001
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973 Program) of China ; National Basic Research Program (973 Program) of China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973 Program) of China ; National Basic Research Program (973 Program) of China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973 Program) of China ; National Basic Research Program (973 Program) of China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973 Program) of China ; National Basic Research Program (973 Program) of China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/14494]
专题	中国科学院武汉物理与数学研究所
通讯作者	Jia, Fan; Xu, Fuqiang
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai, Peoples R China 3.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Wuhan Inst Phys & Math, Wuhan, Hubei, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Phys & Math, Brain Res Ctr, Wuhan, Hubei, Peoples R China 5.Fifth Hosp Wuhan, Dept Orthopaed, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Jia, Fan,Lv, Pei,Miao, Huan,et al. Optimization of the Fluorescent Protein Expression Level Based on Pseudorabies Virus Bartha Strain for Neural Circuit Tracing[J]. FRONTIERS IN NEUROANATOMY,2019,13:9.
APA	Jia, Fan.,Lv, Pei.,Miao, Huan.,Shi, Xiangwei.,Mei, Hongjun.,...&Xu, Fuqiang.(2019).Optimization of the Fluorescent Protein Expression Level Based on Pseudorabies Virus Bartha Strain for Neural Circuit Tracing.FRONTIERS IN NEUROANATOMY,13,9.
MLA	Jia, Fan,et al."Optimization of the Fluorescent Protein Expression Level Based on Pseudorabies Virus Bartha Strain for Neural Circuit Tracing".FRONTIERS IN NEUROANATOMY 13(2019):9.