Analysis of oligonucleotides by ion-pair reversed-phase liquid chromatography coupled with positive mode electrospray ionization mass spectrometry

doi:10.1007/s00216-019-01819-w

	Analysis of oligonucleotides by ion-pair reversed-phase liquid chromatography coupled with positive mode electrospray ionization mass spectrometry
	Weng, Guofeng 2,3; Sun, Binwen 1,3; Liu, Zheyi 1,3; Wang, Fangjun 3; Pan, Yuanjiang 2
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2019-07-01
卷号	411 期号:18 页码:4167-4173
关键词	Oligonucleotide Ion-pair reversed-phase liquid chromatography Positive mode mass spectrometry In-source energy
ISSN号	1618-2642
DOI	10.1007/s00216-019-01819-w
通讯作者	Wang, Fangjun(wangfj@dicp.ac.cn) ; Pan, Yuanjiang(panyuanjiang@zju.edu.cn)
英文摘要	Oligonucleotides are usually analyzed by ion-pair reversed-phase liquid chromatography (IP-RPLC) coupled with negative mode electrospray ionization mass spectrometry (ESI-MS) due to their highly negative charged phosphodiester backbones. Herein, the signal suppression effect of triethylamine (TEA) adducts caused the ion-pair reagent TEA/hexafluoroisopropanol (HFIP) is greatly alleviated after improving the in-source energy in positive mode ESI-MS. This strategy is applied for different RNA sequencing through analyzing their formic acid hydrolysates via IP-RPLC MS. Comparing with negative ion mode, we demonstrate that IP-RPLC MS analysis in positive ion mode is more suitable for RNA sequencing with fewer contaminant interferences. Finally, simultaneous online separation and detection of oligonucleotides and protein digests are achieved in positive ion mode IP-RPLC MS analysis with little interference to each other.
资助项目	National Key R&D Program of China[2016YFF0200503] ; National Natural Science Foundation of China[91853101] ; National Natural Science Foundation of China[21675152] ; National Natural Science Foundation of China[21532005]
WOS关键词	CHEMICALLY-MODIFIED OLIGONUCLEOTIDES ; CROSS-LINKING ; POSTTRANSLATIONAL MODIFICATIONS ; PROTEOMIC ANALYSIS ; CATION-EXCHANGE ; PERFORMANCE ; RNA ; SEPARATION ; ACID ; SUPPRESSION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000474266400021
资助机构	National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/175162]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wang, Fangjun; Pan, Yuanjiang
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Zhejiang Univ, Dept Chem, Hangzhou 310027, Zhejiang, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Weng, Guofeng,Sun, Binwen,Liu, Zheyi,et al. Analysis of oligonucleotides by ion-pair reversed-phase liquid chromatography coupled with positive mode electrospray ionization mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(18):4167-4173.
APA	Weng, Guofeng,Sun, Binwen,Liu, Zheyi,Wang, Fangjun,&Pan, Yuanjiang.(2019).Analysis of oligonucleotides by ion-pair reversed-phase liquid chromatography coupled with positive mode electrospray ionization mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(18),4167-4173.
MLA	Weng, Guofeng,et al."Analysis of oligonucleotides by ion-pair reversed-phase liquid chromatography coupled with positive mode electrospray ionization mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.18(2019):4167-4173.