Purification of tertiary and quaternary alkaloids from Rhizoma Corydalis using reversed-phase/weak cation-exchange mixed-mode class separation combined with preparative C18 and silica based strong cation-exchange chromatography

doi:10.1016/j.jchromb.2019.121742

	Purification of tertiary and quaternary alkaloids from Rhizoma Corydalis using reversed-phase/weak cation-exchange mixed-mode class separation combined with preparative C18 and silica based strong cation-exchange chromatography
	Guo, Xiujie 1,2,4; Gao, Zhenhua 1,3; Wang, Jixia 1,2; Guo, Zhimou 1,2; Zhang, Xiuli 2; Liu, Yanfang 1,2; Wang, Lianzhi 3; Wang, Chaoran1,2 ; Liang, Xinmiao 1,2
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2019-09-15
卷号	1126 页码:7
关键词	Alkaloids Purification C18WCx Scx Rhizoma Corydalis
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2019.121742
通讯作者	Wang, Chaoran(wcrpj_505@dicp.ac.cn) ; Liang, Xinmiao(liangxm@dicp.ac.cn)
英文摘要	A new optimization strategy for purification of alkaloids from Rhizoma Corydalis using preparative liquid chromatography was developed, featuring a selective separation of different types of alkaloids into different parts by a reversed-phase/weak cation-exchange mixed-mode column (named C18WCX) at first. The total alkaloids of Rhizoma Corydalis were divided into four fractions with fraction III and IV corresponding to the tertiary type medium bases and the quaternary type strong bases, respectively. For fraction III, a conventional C18 column was used to isolate tertiary alkaloids using acetonitrile and 0.1% phosphoric acid (adjusted with triethylamine to pH 6.0) as mobile phases. High selectivity and symmetrical peak shapes of tertiary alkaloids were obtained, resulting in six main tertiary alkaloids isolated in a single run. As strong bases, quaternary alkaloids often suffer from serious peak tailing problem on conventional C18 columns. Therefore, a silica-based strong cation-exchange (SCX) column was used for purification of fraction IV. On the SCX column, good peak shapes in high sample loading were achieved. Five main quaternary alkaloids were isolated and identified from the fraction in one-step. The procedures presented effective for the preparative isolation and purification of alkaloids from Rhizoma Corydalis.
资助项目	DICP, CAS[DICP 1201917] ; DICP, CAS[ZZBS201803] ; DICP-CMC Innovation Institute of Medicine
WOS关键词	TRADITIONAL CHINESE MEDICINE ; W. T. WANG ; DL-TETRAHYDROPALMATINE ; YANHUSUO WANG,W.T. ; BASIC COMPOUNDS ; PERFORMANCE ; PLANT
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000488136700006
资助机构	DICP, CAS ; DICP, CAS ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine ; DICP, CAS ; DICP, CAS ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine ; DICP, CAS ; DICP, CAS ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine ; DICP, CAS ; DICP, CAS ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/172514]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wang, Chaoran; Liang, Xinmiao
作者单位	1.DICP CMC Innovat Inst Med, Taizhou 225300, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Hubei Minzu Univ, Sch Chem & Environm Engn, Enshi 445000, Peoples R China 4.Taizhou New Drug Innovat Serv Ctr, Taizhou 225300, Peoples R China
推荐引用方式 GB/T 7714	Guo, Xiujie,Gao, Zhenhua,Wang, Jixia,et al. Purification of tertiary and quaternary alkaloids from Rhizoma Corydalis using reversed-phase/weak cation-exchange mixed-mode class separation combined with preparative C18 and silica based strong cation-exchange chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2019,1126:7.
APA	Guo, Xiujie.,Gao, Zhenhua.,Wang, Jixia.,Guo, Zhimou.,Zhang, Xiuli.,...&Liang, Xinmiao.(2019).Purification of tertiary and quaternary alkaloids from Rhizoma Corydalis using reversed-phase/weak cation-exchange mixed-mode class separation combined with preparative C18 and silica based strong cation-exchange chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1126,7.
MLA	Guo, Xiujie,et al."Purification of tertiary and quaternary alkaloids from Rhizoma Corydalis using reversed-phase/weak cation-exchange mixed-mode class separation combined with preparative C18 and silica based strong cation-exchange chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1126(2019):7.