Molecular cloning and expression analysis of a selenium-independent glutathione peroxidase identified from Manila clam Venerupis philippinarum

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Molecular cloning and expression analysis of a selenium-independent glutathione peroxidase identified from Manila clam Venerupis philippinarum
	Zhang, Lei 1,2,3; Ning, Xuanxuan 4; Chen, Leilei 5; Li, Chenghua 5; Liu, Feng 6; Wang, Qing1,2 ; Wu, Huifeng1,2 ; Zhao, Jianmin1,2
刊名	AQUACULTURE RESEARCH
	2012-07-01
卷号	43 期号:8 页码:1176-1183
关键词	selenium-independent glutathione peroxidase Venerupis philippinarum immune response bacterial challenge
ISSN号	1355-557X
通讯作者	Zhao, JM (reprint author), Chinese Acad Sci, Yantai Coastal Zone Reseach Inst, Key Lab Coastal Zone Environm Proc, 17 Chunhui Rd, Yantai 264003, Peoples R China.,jmzhao@yic.ac.cn
产权排序	[Zhang, Lei; Wang, Qing; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Yantai Coastal Zone Reseach Inst, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Lei; Wang, Qing; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Lei] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Ning, Xuanxuan] Yantai Ocean Environm Monitoring Cent Stn SOA, Yantai, Peoples R China; [Chen, Leilei; Li, Chenghua] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Liu, Feng] China Agr Univ Yantai, Yantai, Peoples R China
中文摘要	Glutathione peroxidase (GPx) is a key enzyme of cellular detoxification systems that defend cells against reactive oxygen species. It can be divided into selenium-dependent GPx (Se-GPx) and selenium-independent GPx (non-Se-GPx) subfamilies. In this study, the full-length cDNA of a non-Se-GPx was identified from Venerupis philippinarum (denoted as VpGPx) using EST analysis and RACE approaches. The full-length cDNA of VpGPx encoded a polypeptide of 226 amino acids with the predicted molecular mass of 25.41 similar to kDa. Phylogenetic analysis indicated that VpGPx had higher evolutional conservation to invertebrate than vertebrate counterparts and should be a new member of the GPx protein family. Spatial expression analysis found that VpGPx transcript was most abundantly expressed in hepatopancreas, gills and haemocytes, and weakly expressed in the tissues of mantle. After Vibrio anguillarum challenge, the expression of VpGPx transcript in overall haemocyte population was down-regulated in the first 6 similar to h, and increased to the peak at 48 similar to h. As time progressed, the expression level dropped to 0.5-fold of the control level at 96 similar to h. All these results indicated that VpGPx was perhaps involved in the immune response against microbe infection and might be contributed to the clearance of bacterial pathogens.
英文摘要	Glutathione peroxidase (GPx) is a key enzyme of cellular detoxification systems that defend cells against reactive oxygen species. It can be divided into selenium-dependent GPx (Se-GPx) and selenium-independent GPx (non-Se-GPx) subfamilies. In this study, the full-length cDNA of a non-Se-GPx was identified from Venerupis philippinarum (denoted as VpGPx) using EST analysis and RACE approaches. The full-length cDNA of VpGPx encoded a polypeptide of 226 amino acids with the predicted molecular mass of 25.41 similar to kDa. Phylogenetic analysis indicated that VpGPx had higher evolutional conservation to invertebrate than vertebrate counterparts and should be a new member of the GPx protein family. Spatial expression analysis found that VpGPx transcript was most abundantly expressed in hepatopancreas, gills and haemocytes, and weakly expressed in the tissues of mantle. After Vibrio anguillarum challenge, the expression of VpGPx transcript in overall haemocyte population was down-regulated in the first 6 similar to h, and increased to the peak at 48 similar to h. As time progressed, the expression level dropped to 0.5-fold of the control level at 96 similar to h. All these results indicated that VpGPx was perhaps involved in the immune response against microbe infection and might be contributed to the clearance of bacterial pathogens.
学科主题	Fisheries
研究领域[WOS]	Fisheries
关键词[WOS]	MARINE-INVERTEBRATES ; OXIDATIVE STRESS ; GENE-EXPRESSION ; REACTIVE OXYGEN ; UNIO-TUMIDUS ; HOMOLOGY ; INFECTION ; SEQUENCE ; DISEASES ; ENZYMES
收录类别	SCI
资助信息	NSFC[30901115]; 100 Talents Program of the Chinese Academy of Sciences; Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology, SOA[201115]
语种	英语
WOS记录号	WOS:000306184700013
公开日期	2013-03-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6017]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Coastal Zone Reseach Inst, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Yantai Ocean Environm Monitoring Cent Stn SOA, Yantai, Peoples R China 5.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 6.China Agr Univ Yantai, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lei,Ning, Xuanxuan,Chen, Leilei,et al. Molecular cloning and expression analysis of a selenium-independent glutathione peroxidase identified from Manila clam Venerupis philippinarum[J]. AQUACULTURE RESEARCH,2012,43(8):1176-1183.
APA	Zhang, Lei.,Ning, Xuanxuan.,Chen, Leilei.,Li, Chenghua.,Liu, Feng.,...&Zhao, Jianmin.(2012).Molecular cloning and expression analysis of a selenium-independent glutathione peroxidase identified from Manila clam Venerupis philippinarum.AQUACULTURE RESEARCH,43(8),1176-1183.
MLA	Zhang, Lei,et al."Molecular cloning and expression analysis of a selenium-independent glutathione peroxidase identified from Manila clam Venerupis philippinarum".AQUACULTURE RESEARCH 43.8(2012):1176-1183.