Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli
	Wang, Xiangwei 1,2; Li, Lingling 1,3; Zheng, Yanning 1,2; Zou, Huibin 1; Cao, Yujin 1; Liu, Hui 1; Liu, Wei 1; Xian, Mo1
刊名	BIORESOURCE TECHNOLOGY
	2012-06-01
卷号	114 期号:114 页码:561-566
关键词	Long chain hydroxyfatty acid Escherichia coli Thioesterase Fatty acid hydroxylase fadD knocking out
英文摘要	This study devised a pathway in Escherichia coli for direct production of long chain hydroxyfatty acids (HFAs) from glucose. This is first report on the biosynthesis of HFAs from renewable sugar, without the need of exogenous fatty acids. By employing thioesterases BTE and 'TesA to tailor the composition of free fatty acids (FFAs) and using fatty acid hydroxylase P450(BM3) to convert FFAs to HFAs, high-specificity production of C12 and C14 HFAs was achieved. By further knocking out the endogenous fadD gene of E. coli, an engineered strain capable of producing 117.0 mg/L HFAs was finally obtained, representing a high HFA production in shake flask. This study indicated an attractive metabolic strategy for the biosynthesis of HFAs directly from renewable carbohydrates resources. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	生物基化学品
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	FATTY-ACIDS ; BACILLUS-MEGATERIUM ; MICROBIAL-PRODUCTION ; OLEIC-ACID ; MONOOXYGENASE ; HYDROXYLATION ; OMEGA ; BIOCATALYSIS ; THIOESTERASE ; RECOMBINANT
收录类别	SCI
语种	英语
WOS记录号	WOS:000305035900079
公开日期	2012-11-07
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1120]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Sichuan Agr Univ, Coll Food Sci, Yaan 625014, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiangwei,Li, Lingling,Zheng, Yanning,et al. Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2012,114(114):561-566.
APA	Wang, Xiangwei.,Li, Lingling.,Zheng, Yanning.,Zou, Huibin.,Cao, Yujin.,...&Xian, Mo.(2012).Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli.BIORESOURCE TECHNOLOGY,114(114),561-566.
MLA	Wang, Xiangwei,et al."Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli".BIORESOURCE TECHNOLOGY 114.114(2012):561-566.