In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells

doi:10.1007/s10811-018-1713-x

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells
	Liu, YingJuan 1,2,3; Deng, Zhenzhen 1,2,3; Geng, Lihua 1,2,3; Wang, Jing1,2 ; Zhang, Quanbin 1,2
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2019-08-01
卷号	31 期号:4 页码:2559-2571
关键词	Rhodophyta Oligo-porphyran Apoptosis 6-OHDA PC12 cells Mitochondria
ISSN号	0921-8971
DOI	10.1007/s10811-018-1713-x
通讯作者	Wang, Jing(jingwang@qdio.ac.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn)
英文摘要	Porphyran, derived from the marine red algae Porphyra and Pyropia, is a type of polysaccharide. Oligo-porphyran (OP) was prepared from porphyran (isolated from Pyropia yezoensis) through the acidolysis reaction containing a linear backbone of alternating 3-linked beta-d-galactose and 4-linked alpha-l-galactose-6-sulfate. Our previous studies have shown that oligo-porphyran could attenuate MPTP-induced behavioral deficits in mice by inhibiting the tyrosine hydroxylase (TH) loss. In the present study, we further investigated how OP protects the dopamine neurons from loss in PC12 cells. OP significantly attenuated LDH release and the production of reactive oxygen species (ROS) lesioned by 6-OHDA. Moreover, OP alleviated the mitochondrial membrane potential (MMP) loss, the ratio of Bax/Bcl-2, and upregulated the PI3K/ Akt/PKC pathway, the level of tyrosine hydroxylase (TH), and dopamine transporter (DAT). Furthermore, OP decreased the production of proinflammatory cytokines. Terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining further indicated that OP treatment inhibited apoptosis. These results might contribute to the overall understanding of the potential health benefits of OP for food and drug applications.
资助项目	National Key RD Plan[2018YFD0901104] ; Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Programme of the Chinese Academy of Sciences[ZSTH-025] ; Natural Science Foundation of China[41406144] ; Natural Science Foundation of China[41506165] ; Ocean Public Welfare Scientific Research Project ; State Oceanic Administration of the People's Republic of China[201405040] ; Science and Technology project of Shandong province[2016GSF115031] ; Youth Innovation Promotion Association of CAS[2016190]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000479057200036
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/162324]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Jing; Zhang, Quanbin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, YingJuan,Deng, Zhenzhen,Geng, Lihua,et al. In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(4):2559-2571.
APA	Liu, YingJuan,Deng, Zhenzhen,Geng, Lihua,Wang, Jing,&Zhang, Quanbin.(2019).In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells.JOURNAL OF APPLIED PHYCOLOGY,31(4),2559-2571.
MLA	Liu, YingJuan,et al."In vitro evaluation of the neuroprotective effect of oligo-porphyran from Porphyra yezoensis in PC12 cells".JOURNAL OF APPLIED PHYCOLOGY 31.4(2019):2559-2571.