Multifunctional alpha-amylase Amy19 possesses agarase, carrageenase, and cellulase activities

doi:10.1016/j.ijbiomac.2018.12.217

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Multifunctional alpha-amylase Amy19 possesses agarase, carrageenase, and cellulase activities
	Li, Jiang 1; Gu, Xiaoqian 1; Pan, Aihong 2
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2019-04
卷号	126 页码:585-594
关键词	Multifunctional enzyme alpha-Amylase Glycosyl hydrolase 70
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2018.12.217
英文摘要	The open reading frame of an alpha-amylase coding gene, amy19, was obtained from a fosmid genomic library of hot spring bacterium Bacillus BI-19 by a plate-based assay of carrageenase activity. After heterologous expression of the gene, the recombinant Amy19 was found to possess alpha-amylase, agarase, carrageenase, and cellulase activities, and could degrade soluble starch, agarose, carrageen, and sodium cellulose into oligosaccharides with low degrees of polymerization. To explore the multifunctional mechanism of Amy19, three continuous glycosyl hydrolase 70 (GH70) motifs in the Amy19 encoding sequence were deleted one by one, then in pairs, then all at once. The GH70 motifs may play an important role in the multifunctionality of Amy19, but the multifunctionality was not determined by the GH70 motifs alone. To our knowledge, this is the first report of an alpha-amylase from a hot spring bacterium with additional agarase, carrageenase, and cellulase activities. (C) 2019 Elsevier B.V. All rights reserved.
资助项目	Public Science and Technology Research Funds Project of Ocean[201505026-4]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000460710000068
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/12773]
专题	业务部门_海洋生态研究中心
通讯作者	Li, Jiang
作者单位	1.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China 2.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China
推荐引用方式 GB/T 7714	Li, Jiang,Gu, Xiaoqian,Pan, Aihong. Multifunctional alpha-amylase Amy19 possesses agarase, carrageenase, and cellulase activities[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,126:585-594.
APA	Li, Jiang,Gu, Xiaoqian,&Pan, Aihong.(2019).Multifunctional alpha-amylase Amy19 possesses agarase, carrageenase, and cellulase activities.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,126,585-594.
MLA	Li, Jiang,et al."Multifunctional alpha-amylase Amy19 possesses agarase, carrageenase, and cellulase activities".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 126(2019):585-594.