Simultaneous regulation of F5H in COMT-RNAi transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis

doi:10.1111/pbi.13019

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Simultaneous regulation of F5H in COMT-RNAi transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis
	Wu, Zhenying 1; Wang, Nengfei 2; Hisano, Hiroshi 3,4; Cao, Yingping 1; Wu, Fengyan 3; Liu, Wenwen 1; Bao, Yan 1; Wang, Zeng-Yu 3; Fu, Chunxiang 1
刊名	PLANT BIOTECHNOLOGY JOURNAL
	2019-04
卷号	17 期号:4 页码:836-845
关键词	caffeic acid O-methyltransferase coordinated effects ferulate 5-hydroxylase lignin biosynthesis Panicum virgatum L switchgrass
ISSN号	1467-7644
DOI	10.1111/pbi.13019
英文摘要	Ferulate 5-hydroxylase (F5H) catalyses the hydroxylation of coniferyl alcohol and coniferaldehyde for the biosynthesis of syringyl (S) lignin in angiosperms. However, the coordinated effects of F5H with caffeic acid O-methyltransferase (COMT) on the metabolic flux towards S units are largely unknown. We concomitantly regulated F5H expression in COMT-down-regulated transgenic switchgrass (Panicum virgatum L.) lines and studied the coordination of F5H and COMT in lignin biosynthesis. Down-regulation of F5H in COMT-RNAi transgenic switchgrass plants further impeded S lignin biosynthesis and, consequently, increased guaiacyl (G) units and reduced 5-OH G units. Conversely, overexpression of F5H in COMT-RNAi transgenic plants reduced G units and increased 5-OH units, whereas the deficiency of S lignin biosynthesis was partially compensated or fully restored, depending on the extent of COMT down-regulation in switchgrass. Moreover, simultaneous regulation of F5H and COMT expression had different effects on cell wall digestibility of switchgrass without biomass loss. Our results indicate that up-regulation and down-regulation of F5H expression, respectively, have antagonistic and synergistic effects on the reduction in S lignin resulting from COMT suppression. The coordinated effects between lignin genes should be taken into account in future studies aimed at cell wall bioengineering.
资助项目	Major Program of Shandong Province Natural Science Foundation[ZR2018ZB0213]
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
语种	英语
出版者	WILEY
WOS记录号	WOS:000461859900014
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/12730]
专题	业务部门_海洋生态研究中心
通讯作者	Fu, Chunxiang
作者单位	1.Chinese Acad Sci, Qingdao Inst Thoenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao, Shandong, Peoples R China 2.State Ocean Adm, Key Lab Marine Bioact Subst, Inst Oceanog 1, Qingdao, Shandong, Peoples R China 3.Noble Res Inst LLC, Ardmore, OK USA 4.Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama, Japan
推荐引用方式 GB/T 7714	Wu, Zhenying,Wang, Nengfei,Hisano, Hiroshi,et al. Simultaneous regulation of F5H in COMT-RNAi transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis[J]. PLANT BIOTECHNOLOGY JOURNAL,2019,17(4):836-845.
APA	Wu, Zhenying.,Wang, Nengfei.,Hisano, Hiroshi.,Cao, Yingping.,Wu, Fengyan.,...&Fu, Chunxiang.(2019).Simultaneous regulation of F5H in COMT-RNAi transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis.PLANT BIOTECHNOLOGY JOURNAL,17(4),836-845.
MLA	Wu, Zhenying,et al."Simultaneous regulation of F5H in COMT-RNAi transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis".PLANT BIOTECHNOLOGY JOURNAL 17.4(2019):836-845.