Simultaneous regulation of F5H in COMT-RNAi transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis | |
Wu, Zhenying1; Wang, Nengfei2; Hisano, Hiroshi3,4; Cao, Yingping1; Wu, Fengyan3; Liu, Wenwen1; Bao, Yan1; Wang, Zeng-Yu3; Fu, Chunxiang1 | |
刊名 | PLANT BIOTECHNOLOGY JOURNAL |
2019-04 | |
卷号 | 17期号:4页码:836-845 |
关键词 | caffeic acid O-methyltransferase coordinated effects ferulate 5-hydroxylase lignin biosynthesis Panicum virgatum L switchgrass |
ISSN号 | 1467-7644 |
DOI | 10.1111/pbi.13019 |
英文摘要 | Ferulate 5-hydroxylase (F5H) catalyses the hydroxylation of coniferyl alcohol and coniferaldehyde for the biosynthesis of syringyl (S) lignin in angiosperms. However, the coordinated effects of F5H with caffeic acid O-methyltransferase (COMT) on the metabolic flux towards S units are largely unknown. We concomitantly regulated F5H expression in COMT-down-regulated transgenic switchgrass (Panicum virgatum L.) lines and studied the coordination of F5H and COMT in lignin biosynthesis. Down-regulation of F5H in COMT-RNAi transgenic switchgrass plants further impeded S lignin biosynthesis and, consequently, increased guaiacyl (G) units and reduced 5-OH G units. Conversely, overexpression of F5H in COMT-RNAi transgenic plants reduced G units and increased 5-OH units, whereas the deficiency of S lignin biosynthesis was partially compensated or fully restored, depending on the extent of COMT down-regulation in switchgrass. Moreover, simultaneous regulation of F5H and COMT expression had different effects on cell wall digestibility of switchgrass without biomass loss. Our results indicate that up-regulation and down-regulation of F5H expression, respectively, have antagonistic and synergistic effects on the reduction in S lignin resulting from COMT suppression. The coordinated effects between lignin genes should be taken into account in future studies aimed at cell wall bioengineering. |
资助项目 | Major Program of Shandong Province Natural Science Foundation[ZR2018ZB0213] |
WOS关键词 | DOWN-REGULATION ; CELL-WALL ; REDUCES RECALCITRANCE ; ARABIDOPSIS ; EXPRESSION ; MANIPULATION ; DEFICIENT ; PLANTS ; IMPROVEMENT ; ALCOHOL |
WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
语种 | 英语 |
出版者 | WILEY |
WOS记录号 | WOS:000461859900014 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn:8080/handle/2SI8HI0U/24808] |
专题 | 自然资源部第一海洋研究所 |
通讯作者 | Fu, Chunxiang |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Thoenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao, Shandong, Peoples R China 2.State Ocean Adm, Key Lab Marine Bioact Subst, Inst Oceanog 1, Qingdao, Shandong, Peoples R China 3.Noble Res Inst LLC, Ardmore, OK USA 4.Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama, Japan |
推荐引用方式 GB/T 7714 | Wu, Zhenying,Wang, Nengfei,Hisano, Hiroshi,et al. Simultaneous regulation of F5H in COMT-RNAi transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis[J]. PLANT BIOTECHNOLOGY JOURNAL,2019,17(4):836-845. |
APA | Wu, Zhenying.,Wang, Nengfei.,Hisano, Hiroshi.,Cao, Yingping.,Wu, Fengyan.,...&Fu, Chunxiang.(2019).Simultaneous regulation of F5H in COMT-RNAi transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis.PLANT BIOTECHNOLOGY JOURNAL,17(4),836-845. |
MLA | Wu, Zhenying,et al."Simultaneous regulation of F5H in COMT-RNAi transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis".PLANT BIOTECHNOLOGY JOURNAL 17.4(2019):836-845. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论