Chitosan Functionalized CuS Nanoparticles Boots Gene Transfection via   Photothermal Effect

doi:10.2174/1567201813666160108114430

CORC > 北京大学 > 工学院

	Chitosan Functionalized CuS Nanoparticles Boots Gene Transfection via Photothermal Effect
	Lin, Li ; Li, Xiaoda ; Yang, Yongbo ; Jing, Lijia ; Yue, Xiuli ; Chen, Xuzhu ; Dai, Zhifei
刊名	CURRENT DRUG DELIVERY
	2017
关键词	Biocompatibility chitosan copper sulfide gene transfection photocontrollable nanovector photothermal effect COPPER SULFIDE NANOPARTICLES BLOOD-BRAIN-BARRIER IN-VITRO TARGETED DELIVERY THERAPY VIVO CANCER SIRNA ABLATION CARRIER
DOI	10.2174/1567201813666160108114430
英文摘要	Background: The lack of smart and controllable gene vectors with high safety and efficiency is still a main obstruction for clinical applications of gene therapy. Recently, the external physical stimuli, such as near infrared light induced temperature elevation, have been applied to enhance the gene transfection efficiency and specificity. The aim of this paper is to fabricate chitosan functionalized CuS nanoparticles (CuS@CS NPs) with small size and higher biocompatibility for enhanced gene delivery by photothermal effect. Methods: CuS@CS NPs were successfully prepared by simple hydrothermal method. The biocompatibility was detected by MTT method and hymolytic analysis. pEGFP-Cl was used as gene model, and its expression efficiency was detected by fluorescence microscopy and flow cytometry to investigate the effect of photothermal effect on the transfection efficiency. Results: The CuS@CS NPs around 15 nm were successfully engineered. The modification of CuS nanoparticles with chitosan conduced to higher physiological stability and biocompatibility. The utilization of CuS@CS NPs in combination with external near infrared (NIR) laser irradiation could enhance gene transfection efficiency due to photothermal effect. The gene transfection efficiency of CuS@CS NPs found to increase from 5.05 +/- 10.54% (0 min) to 23.47 +/- 11.27% (10 min), significantly higher than the free polyethylenimine (18.15 +/- 11.03%). Conclusion: CuS@CS NPs showed great capability to control gene delivery by an external NIR laser irradiation and enhance the gene transfection efficiency and specificity because of convenient preparation, stabilized optical properties, excellent photothermal effect and good biocompatibility. It encourages further exploration of the CuS@CS NPs as a photocontrollable nanovector for combined photothermal and gene therapy, as well as image guided therapy.; National Natural Science Foundation of China [81201186, 21273014]; National Natural Science Foundation for Distinguished Young Scholars [81225011]; State Key Program of National Natural Science of China [81230036]; Foundation for Innovative Research Groups of the National Natural Science Foundation of China [81421004]; SCI(E); ARTICLE; 3; 334-341; 14
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/476054]
专题	工学院
推荐引用方式 GB/T 7714	Lin, Li,Li, Xiaoda,Yang, Yongbo,et al. Chitosan Functionalized CuS Nanoparticles Boots Gene Transfection via Photothermal Effect[J]. CURRENT DRUG DELIVERY,2017.
APA	Lin, Li.,Li, Xiaoda.,Yang, Yongbo.,Jing, Lijia.,Yue, Xiuli.,...&Dai, Zhifei.(2017).Chitosan Functionalized CuS Nanoparticles Boots Gene Transfection via Photothermal Effect.CURRENT DRUG DELIVERY.
MLA	Lin, Li,et al."Chitosan Functionalized CuS Nanoparticles Boots Gene Transfection via Photothermal Effect".CURRENT DRUG DELIVERY (2017).