Utilization of cane molasses towards cost-saving astaxanthin production   by a Chlorella zofingiensis mutant

doi:10.1007/s10811-013-9974-x

CORC > 北京大学 > 工学院

	Utilization of cane molasses towards cost-saving astaxanthin production by a Chlorella zofingiensis mutant
	Liu, Jin ; Sun, Zheng ; Zhong, Yujuang ; Gerken, Henri ; Huang, Junchao ; Chen, Feng
刊名	journal of applied phycology
	2013
关键词	Astaxanthin Chlorella zofingiensis mutant Fed-batch Molasses Semi-continuous HAEMATOCOCCUS-PLUVIALIS BIODIESEL PRODUCTION ACID PRODUCTION MICROALGAE FERMENTATION ACCUMULATION GROWTH BIOSYNTHESIS DESATURASE NUTRITION
DOI	10.1007/s10811-013-9974-x
英文摘要	The aim of the present study was to survey the growth and astaxanthin production of E17, an astaxanthin-rich mutant of Chlorella zofingiensis, through feeding the low-cost carbon source cane molasses. In heterotrophic batch cultivation, E17 fed with pretreated molasses achieved biomass (1.79 g L-1 day(-1)) and astaxanthin (1.99 mg L-1 day(-1)) productivities comparable to those with glucose, which were about 2- and 2.8-fold of those fed with untreated molasses, respectively. Molasses-induced astaxanthin accumulation may be attributed to the elicited expression of carotenogenic genes, in particular the genes specifically responsible for the ketolation and hydroxylation of beta-carotene to form astaxanthin. A two-stage fed-batch strategy was employed to grow E17 and induce astaxathin accumulation, resulting in 45.6 g L-1 biomass and 56.1 mg L-1 astaxanthin, the highest volumetric astaxanthin yield ever reported for this alga. In addition, the astaxanthin production by E17 was tested with a semi-continuous culture method, where the directly diluted raw molasses (giving 5 g L-1 sugar) was used as the carbon source. Little growth inhibition of E17 was observed in the semi-continuous culture with a biomass productivity of 1.33 g L-1 day(-1) and an astaxanthin productivity of 0.83 mg L-1 day(-1). The mixotrophic semi-continuous cultures enhanced the biomass and astaxanthin productivities by 29.3 % and 42.2 %, respectively. This study highlights the potential of using the industrially cheap cane molasses towards large-scale cost-saving production of the high-value ketocarotenoid astaxanthin.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000324104700021&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biotechnology & Applied Microbiology; Marine & Freshwater Biology; SCI(E); 5; ARTICLE; 5; 1447-1456; 25
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/154414]
专题	工学院
推荐引用方式 GB/T 7714	Liu, Jin,Sun, Zheng,Zhong, Yujuang,et al. Utilization of cane molasses towards cost-saving astaxanthin production by a Chlorella zofingiensis mutant[J]. journal of applied phycology,2013.
APA	Liu, Jin,Sun, Zheng,Zhong, Yujuang,Gerken, Henri,Huang, Junchao,&Chen, Feng.(2013).Utilization of cane molasses towards cost-saving astaxanthin production by a Chlorella zofingiensis mutant.journal of applied phycology.
MLA	Liu, Jin,et al."Utilization of cane molasses towards cost-saving astaxanthin production by a Chlorella zofingiensis mutant".journal of applied phycology (2013).