Mono-2-ethylhexyl phthalate inhibits human extravillous trophoblast   invasion via the PPAR gamma pathway

doi:10.1016/j.taap.2017.04.014

CORC > 北京大学 > 城市与环境学院

	Mono-2-ethylhexyl phthalate inhibits human extravillous trophoblast invasion via the PPAR gamma pathway
	Gao, Fumei ; Hu, Wenxin ; Li, Yu ; Shen, Huan ; Hu, Jianying
刊名	TOXICOLOGY AND APPLIED PHARMACOLOGY
	2017
关键词	Early pregnancy loss Trophoblast invasion Matrix metalloproteinase Tissue inhibitor of matrix metalloproteinase Phthalates PPAR gamma PREGNANCY LOSS DI(2-ETHYLHEXYL) PHTHALATE OXIDATIVE STRESS CANCER-CELLS METABOLITES EXPOSURE ACTIVATION MIGRATION HEALTH URINE
DOI	10.1016/j.taap.2017.04.014
英文摘要	Concerns over the adverse reproductive outcomes in human have been raised, more evidence including the underlying mechanism are required. Since extravillous trophoblast (EVT) invasion is an important physiological step during early development, the effects of mono-2-ethylhexyl phthalate (MEHP), the bioactive metabolite of DEHP, on EVT invasion were investigated using Matrigel-coated transwell chambers and cell line HTR-8/SVneo. In the transwell-based invasive assay, MEHP exposure inhibited EVT invasion as judged by decreased invasion index. Further analysis showed that MEHP exposure significantly inhibited the activity of matrix metalloproteinase-9 (MMP-9), which is an important positive regulator of EVT invasion. Meanwhile, the protein levels of tissue inhibitor matrix metalloproteinase-1 (TIMP-1), one key negative regulator of EVT invasion, were upregulated by MEHP treatment. Finally, inactivation of PPAR gamma pathway by either PPAR gamma inhibitors or PPAR gamma shRNA knockdown rescued the MEHP-induced inhibited invasion of HTR-8/SVneo cells, which is accompanied by the recovery of inhibited MMP-9 expression. The present study provides the evidence that MEHP exposure inhibits trophoblast invasion via PPAR gamma at concentrations comparable to those found in humans, which provides an insight in understanding the mechanisms of DEHP-associated early pregnancy loss. (C) 2017 Elsevier Inc. All rights reserved.; National Natural Science Foundation of China [21507003, 41330637]; National Special Funding Project for Water Pollution Control and Management of China [2014ZX07405001]; SCI(E); ARTICLE; 23-29; 327
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472124]
专题	城市与环境学院
推荐引用方式 GB/T 7714	Gao, Fumei,Hu, Wenxin,Li, Yu,et al. Mono-2-ethylhexyl phthalate inhibits human extravillous trophoblast invasion via the PPAR gamma pathway[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2017.
APA	Gao, Fumei,Hu, Wenxin,Li, Yu,Shen, Huan,&Hu, Jianying.(2017).Mono-2-ethylhexyl phthalate inhibits human extravillous trophoblast invasion via the PPAR gamma pathway.TOXICOLOGY AND APPLIED PHARMACOLOGY.
MLA	Gao, Fumei,et al."Mono-2-ethylhexyl phthalate inhibits human extravillous trophoblast invasion via the PPAR gamma pathway".TOXICOLOGY AND APPLIED PHARMACOLOGY (2017).