Quantitative proteomics reveals the key molecular events occurring at different cell cycle phases of the in situ blooming dinoflagellate cells

doi:10.1016/j.scitotenv.2019.04.216

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	Quantitative proteomics reveals the key molecular events occurring at different cell cycle phases of the in situ blooming dinoflagellate cells
	Liu, Jiuling 1; Zhang, Hao ; Liu, Siqi 4; Lin, Lin ; Zhang, Shufei 3; Zhang, Yong ; Wang, Dazhi ; Xie, Zhangxian ; He, Yanbin 4
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2019
卷号	676 页码:62
关键词	Cell cycle Dinoflagellates Harmful algal blooms iTRAQ Quantitative proteomics
ISSN号	0048-9697
DOI	10.1016/j.scitotenv.2019.04.216
英文摘要	Dinoflagellate blooms are the results of rapid cell proliferation governed by cell cycle, a highly-ordered series of events that culminates in cell division. However, little is known about cell cycle progression of the in situ bloom cells. Here, we compared proteomes of the in situ blooming cells of a dinoflagellate Prorocentrum donghaiense collected at different cell cycle phases. The blooming P. donghaiense cells completed a cell cycle within 24 h with a high synchronization rate of 82.7%. Proteins associated with photosynthesis, porphyrin and chlorophyll synthesis, carbon, nitrogen and amino add metabolisms exhibited high expressions at the G(1) phase; DNA replication and mismatch repair related proteins were more abundant at the S phase; while protein synthesis and oxidative phosphorylation were highly enriched at the G(2)/M phase. Cell cycle proteins presented similar periodic did patterns to other eukaryotic cells, and higher expressions of proliferating cell nuclear antigen and cyclin dependent kinase 2 at the S phase ensured the smooth S-G(2)/M transition. Strikingly, four histones were first identified in P. donghaiense and highly expressed at the G(2)/M phase, indicating their potential roles in regulating cell cycle. This study presents the first quantitative survey, to our knowledge, of proteome changes at different cell cycle phases of the in situ blooming cells in natural environment and provides insights into cell cycle regulation of the blooming dinoflagellate cells. (C) 2019 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17944]
专题	南海海洋研究所_中科院边缘海地质重点实验室
作者单位	1.Xiamen Univ, State Key Lab Marine Environm Sci, Coll Environm & Ecol, Xiamen 361005, Peoples R China 2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China 3.BGI Shenzhen, Beishan Ind Zone 11th Bldg, Shenzhen 518083, Guangdong, Peoples R China 4.Chinese Acad Sci, Key Lab Ocean & Marginal Sea Geol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jiuling,Zhang, Hao,Liu, Siqi,et al. Quantitative proteomics reveals the key molecular events occurring at different cell cycle phases of the in situ blooming dinoflagellate cells[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,676:62, 71.
APA	Liu, Jiuling.,Zhang, Hao.,Liu, Siqi.,Lin, Lin.,Zhang, Shufei.,...&He, Yanbin.(2019).Quantitative proteomics reveals the key molecular events occurring at different cell cycle phases of the in situ blooming dinoflagellate cells.SCIENCE OF THE TOTAL ENVIRONMENT,676,62.
MLA	Liu, Jiuling,et al."Quantitative proteomics reveals the key molecular events occurring at different cell cycle phases of the in situ blooming dinoflagellate cells".SCIENCE OF THE TOTAL ENVIRONMENT 676(2019):62.