Impact of long-term phosphorus fertilizer inputs on bacterial phoD gene community in a maize field, Northeast China

doi:10.1016/j.scitotenv.2019.03.172

	Impact of long-term phosphorus fertilizer inputs on bacterial phoD gene community in a maize field, Northeast China
	Chen, Xiaodong 1,2; Jiang, Nan 1; Condron, Leo M.3; Dunfield, Kari E.4; Chen, Zhenhua 1; Wang, Jingkuan 5; Chen, Lijun 1
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2019-06-15
卷号	669 页码:1011-1018
关键词	Alkaline phosphomonoesterase activity Soil phosphorus gradient phoD gene abundance and community structure High-throughput sequencing P availability
ISSN号	0048-9697
DOI	10.1016/j.scitotenv.2019.03.172
英文摘要	The bacterial phoD gene encodes alkaline phosphomonoesterase, an enzyme which plays an important role in the release of plant-available inorganic phosphorus (P) from organic P in soil. However, the relationships between phoD gene community, alkaline phosphomonoesterase activity, and P availability in soil are poorly understood. In this study, we investigated how alkaline phosphomonoesterase activity, phoD gene abundance, and community structure are influenced by plant-available P using soils (0-10, 10-20 and 20-40 cm) from a long-term field trial in which a continuous maize (Zea mays L.) crop had received different levels of P fertilizer inputs (30, 60 kg P ha(-1) year(-1)) for 28 years. Quantitative PCR and high-throughput sequencing were used to analyze phoD gene abundance and community composition. Alkaline phosphomonoesterase enzyme activity was negatively correlated with soil available P, which was reflected in corresponding data for phoD gene abundance. On the other hand, positive correlations were determined between phoD gene alpha-diversity and available P, while shifts in phoD gene community structure were related to changes in soil pH and P availability. The relative abundance of Pseudomonas was negatively correlated with P availability and positively correlated with alkaline phosphomonoesterase activity, suggesting that Pseudomonas may play an important role in soil organic P mineralization. The findings of this study demonstrated that changes of soil P availability as a result of long-term P fertilizer inputs significantly affected alkaline phosphomonoesterase activity by regulating phoD gene abundance, diversity, as well as altering the phoD gene community composition. (C) 2019 Published by Elsevier B.V.
资助项目	National Natural Science Foundation of China[41471217] ; National Key Research and Development Program of China[2016YFD0300802]
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000463663500098
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/124033]
专题	中国科学院沈阳应用生态研究所
通讯作者	Chen, Lijun
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, POB 417, Shenyang 110016, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Lincoln Univ, Fac Agr & Life Sci, POB 85084, Christchurch 7647, New Zealand 4.Univ Guelph, Sch Environm Sci, 50 Stone Rd East, Guelph, ON N1G 2W1, Canada 5.Shenyang Agr Univ, Land & Environm Coll, Shenyang 110866, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xiaodong,Jiang, Nan,Condron, Leo M.,et al. Impact of long-term phosphorus fertilizer inputs on bacterial phoD gene community in a maize field, Northeast China[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,669:1011-1018.
APA	Chen, Xiaodong.,Jiang, Nan.,Condron, Leo M..,Dunfield, Kari E..,Chen, Zhenhua.,...&Chen, Lijun.(2019).Impact of long-term phosphorus fertilizer inputs on bacterial phoD gene community in a maize field, Northeast China.SCIENCE OF THE TOTAL ENVIRONMENT,669,1011-1018.
MLA	Chen, Xiaodong,et al."Impact of long-term phosphorus fertilizer inputs on bacterial phoD gene community in a maize field, Northeast China".SCIENCE OF THE TOTAL ENVIRONMENT 669(2019):1011-1018.