A hypervariable immunoglobulin superfamily member from Crassostrea gigas functions as pattern recognition receptor with opsonic activity

doi:10.1016/j.dci.2018.05.007

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	A hypervariable immunoglobulin superfamily member from Crassostrea gigas functions as pattern recognition receptor with opsonic activity
	Liu, Dongyang 1,3; Yi, Qilin 1,3; Wu, Yichen 1,3; Lu, Guangxia 1,3; Gong, Changhao 1,3; Song, Xiaorui1,3 ; Sun, Jiejie 1,3; Qu, Chen 1,3; Liu, Conghui; Wang, Lingling 1,2,3,4
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2018-09-01
卷号	86 页码:96-108
关键词	Crassostrea gigas IgSF Opsonin Recombination Balancing selection
ISSN号	0145-305X
DOI	10.1016/j.dci.2018.05.007
通讯作者	Song, Linsheng(lshsong@dlou.edu.cn)
英文摘要	lmmunoglobulin superfamily (IgSF), an extensive collection of proteins possessing at least one immunoglobulin-like (Ig-like) domain, performs a wide range of functions in recognition, binding or adhesion process of cells. In the present study, a cysteine-rich motif associated immunoglobulin domain containing protein (designated CgCAICP-1) was identified in Pacific oyster Crassostrea gigas. The deduced protein sequence of CgCAICP-1 contained 534 amino acidresidues, with three Ig domains which were designated as IG1, IG2 and IG3, and a cysteine-rich motif between the first and second Ig domain. The mRNA transcripts of CgCAICP-1 were highly expressed in hemocytes and up-regulated significantly (p < 0.05) after the stimulation of Iipopolysaccharides (LPS), but not peptidoglycan (PGN). The recombinant CgCAICP-1 protein (rCgCAICP-1) exhibited binding activity to various pathogen-associated molecular patterns (PAMPs) including LPS, PGN, mannose (Man) and D-galactose (D-Gal), and microorganisms including Vibrio splendidus, Escherichia coli, Staphylococcus aureus, Micrococcus luteus and Pichia pastoris. The phagocytic rates of oyster hemocytes towards Gram-negative bacteria V. splendidus and Gram-positive bacteria M. luteus were significantly enhanced (p < 0.05) after pre-incubation of microbes with rCgCAICP-1. Furthermore, the transcripts of CgCAICP-1 exhibited high level of polymorphism among individuals. The ratio of nonsynonymous and synonymous distances (dN/dS) for AA'BCC'D strands of IG1 (the possible binding sites 1, pbs1) across all allelic variants was 2.09 (p < 0.05), while the ratio for the non-pbs regions was less than 1.0. The 1248 bp fragment amplified from the 5' end of CgCAICP-1 open reading frame (ORF) from 24 transcript variants could be divided artificially into seven regions of 50 elements, and all of the allelic variants might be derived from these elements by point mutation and recombination processes. These results collectively suggested that CgCAICP-1 might function as an important pattern recognition receptor (PRR) to recognize various PAMPs and facilitated the phagocytosis of oyster hemocytes towards both Gram-positive and Gram-negative bacteria. Diverse isoforms of CgCAICP-1 were generated through point mutation and recombination processes and maintained by balancing selection, which would provide a broader spectrum of interaction surface and be associated with immune resistance of oysters to infectious pathogens. (C) 2018 Elsevier Ltd. All rights reserved.
资助项目	National Science Foundation of China[U1706204] ; China Agriculture Research System[CARS-49] ; AoShan Talents Cultivation Program - Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University ; Outstanding Talents and Innovative Teams of Agricultural Scientific Research in the Ministry of Agriculture
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000435060900012
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/159374]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Song, Linsheng
作者单位	1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China 3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Liu, Dongyang,Yi, Qilin,Wu, Yichen,et al. A hypervariable immunoglobulin superfamily member from Crassostrea gigas functions as pattern recognition receptor with opsonic activity[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,86:96-108.
APA	Liu, Dongyang.,Yi, Qilin.,Wu, Yichen.,Lu, Guangxia.,Gong, Changhao.,...&Song, Linsheng.(2018).A hypervariable immunoglobulin superfamily member from Crassostrea gigas functions as pattern recognition receptor with opsonic activity.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,86,96-108.
MLA	Liu, Dongyang,et al."A hypervariable immunoglobulin superfamily member from Crassostrea gigas functions as pattern recognition receptor with opsonic activity".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 86(2018):96-108.