Transcriptome sequencing reveals the involvement of reactive oxygen species in the hematopoiesis from Chinese mitten crab Eriocheir sinensis

doi:10.1016/j.dci.2017.12.030

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Transcriptome sequencing reveals the involvement of reactive oxygen species in the hematopoiesis from Chinese mitten crab Eriocheir sinensis
	Jia, Zhihao 1,3; Wang, Mengqiang1 ; Wang, Xiudan1,3 ; Wang, Lingling 2; Qiu, Limei 1; Song, Linsheng 2,4
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2018-05-01
卷号	82 页码:94-103
关键词	Eriocheir sinensis Reactive oxygen species (ROS) Hematopoietic tissue Transcriptome sequencing Mitochondrial complexes I N-acetyl-L-cysteine (NAC)
ISSN号	0145-305X
DOI	10.1016/j.dci.2017.12.030
通讯作者	Qiu, Limei(qiulimei@qdio.ac.cn) ; Song, Linsheng(lshsong@qdio.ac.cn)
英文摘要	Reactive oxygen species (ROS) produced in vivo during various electron transfer reactions are generally kept at a certain level since they are harmful to cells. However, it can sensitize hematopoietic progenitors to differentiation, and plays a signaling role in the regulation of hematopoietic cell fate. In the present study, the transcriptomes of crab HPT and hemocytes were sequenced using the Ion Torrent Proton sequencing platform. A total of 51,229,690 single end reads were obtained from six single-end libraries, which were assembled into 31346 unireads as reference. After mapping and transcript assembling, 362 differently expressed genes were identified and 301 of them were deemed to be more abundant in HPT. GO annotation revealed that they were mostly implicated in DNA, RNA and protein synthesis, cell division, mitochondria activities and energy metabolism. The expression level of mitochondrial complexes I (mitochondrial NADH-ubiquinone oxidoreductase) which was the main natural producers of mitochondrial ROS was found to be 8.6-fold (p < 0.01) higher in HPT than that in hemocytes. In hemocytes, the proteinase genes associated with proPO activation from the 61 up-regulated genes in hemocytes were the main up-regulated genes which might be the potential markers for mature hemocytes. ROS level in HPT cells was relatively higher which was confirmed with the high expression level of mitochondria related genes identified by transcriptome sequencing. After the ROS level was depressed by N-acetyk-cysteine (NAC), the production of hemocytes from HPT was inhibited, and the recovery of the total hemocytes counts was delayed. These results collectively indicated that the genes in redox system were more active in HPT, and ROS could function as an important modulator in the hematopoiesis of crab and promote the production of hemocytes from HPT. (C) 2018 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[31530069] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian high level talent innovation support program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49] ; AoShan Talents Cultivation Program
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000427668700010
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158396]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Qiu, Limei; Song, Linsheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jia, Zhihao,Wang, Mengqiang,Wang, Xiudan,et al. Transcriptome sequencing reveals the involvement of reactive oxygen species in the hematopoiesis from Chinese mitten crab Eriocheir sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,82:94-103.
APA	Jia, Zhihao,Wang, Mengqiang,Wang, Xiudan,Wang, Lingling,Qiu, Limei,&Song, Linsheng.(2018).Transcriptome sequencing reveals the involvement of reactive oxygen species in the hematopoiesis from Chinese mitten crab Eriocheir sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,82,94-103.
MLA	Jia, Zhihao,et al."Transcriptome sequencing reveals the involvement of reactive oxygen species in the hematopoiesis from Chinese mitten crab Eriocheir sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 82(2018):94-103.