Mor-platin a new platinum complex that can induce apoptosis and inhibit migration

doi:10.1360/N972016-00561

	Mor-platin a new platinum complex that can induce apoptosis and inhibit migration
	Li, Jingling ; Chen CY(陈春英); Cao, Jianping ; Zhang, Leshuai ; Guo, Zhengqing ; Chen, Chunying
刊名	Chinese Science Bulletin科学通报
	2017
卷号	62 期号:4 页码:270-278
ISSN号	0023-074X
DOI	10.1360/N972016-00561
其他题名	新型铂配合物Mor-platin导致细胞凋亡及抑制细胞迁移
文献子类	Article
英文摘要	Since cisplatin was discovered and applied clinically, people have synthesized thousands of platinum compound derivatives. These platinum derivatives are effective in chemotherapy for some tumors including testicular cancer, ovarian cancer, lung cancer, bladder cancer, head and neck cancer. All of these platinum complex are believed to exert their unparalleled anticancer potency by interacting with nulear DNA, resulting in DNA adducts formation and consequently lead to cell apoptosis, cell cycle arrest and reactive oxygen species released. However, these platinum complex are severely limited by drug resistance and undesirable side effects arising from relatively low specificity and resultant high dosage requirement. To overcome the drug-resistance and improve therapeutic efficiency, it is necessary to synthesized new platinum complex. Our group successfully synthesized a new platinum complex Mor-platin, it can significantly damage more tumor cells than traditional drug cisplatin, while the other biological effects have not been studied. This work aims to study the effect of Mor-platin on its anti-cancer ability, cell migration and cell invasion. In order to detect anti-cancer efficiency, MTT assay was used, and the results showed that IC50of Mor-platin (14.41 μg mL-1) was far less than IC50of cisplatin (97.29 μg mL-1), suggesting that Mor-platin have potentially higher tumor cytotoxicity than cisplatin. Next, we used reactive oxygen species assay kit, western blot analysis, apoptosis and cell cycle kit to assess whether Mor-platin can cause cell death by apoptosis, cell cycle, ROS, apoptosis-related protein and caspase protein. The results showed that Mor-platin can increase level of ROS, arrest cell cyle in S phase, increase ratio of Bax/Bcl-2 and activate caspase 3/8/9 resulting in cleaved PARP, ultimately lead to cell death. The major threat of cancer is not from the primary tumor itself but from metastasis to other organs. To develop successful metastases, cancer cells need to disseminate to a distant organ, migrate out of the primary tumor, invade to blood or lymphatic vessels, finally seed, proliferate and colonize to form secondary tumors. Cell migration is a basic process within the cancer. Tumor metastasis is a major cause of cancer deaths, 90% of the cancer deaths can be attributed to the tumor metastasis, therefore it is necessary to avoid tumor metastasis on clinical cancer treatment. Only understanding the clear tumor metastasis for emerging antitumor drugs, they can be obtained to development and application. Current tumor metastasis experiments include transwell assay, wound-healing assay, microcarrier beads, three-dimensional assay and so on. Here, we used wound-healing assay and transwell assay to quantify the migration inhibitory activity of Mor-platin. Intriguingly, both wound-healing assay and transwell assay showed that Mor-platin can also inhibit cell migration and invasion, while cisplatin did not show any same effects. In summary, this new platinum complex Mor-platin not only have high anti-cancer efficiency but also inhibit migration, providing a novel strategy for the development of next generation platinum complex with optimal anti-tumor activities. © 2017, Science Press. All right reserved.
电子版国际标准刊号	2095-9419
语种	中文
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/285457]
专题	高能物理研究所_多学科研究中心
作者单位	1.Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China and Institute of High Energy Physics, Chinese Academy of Sciences, Beijing; 100049, China 2.Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, School of Radiation Medicine and Protection, Medical College of Soochow University, Soochow; 215123, China;
推荐引用方式 GB/T 7714	Li, Jingling,Chen CY,Cao, Jianping,et al. Mor-platin a new platinum complex that can induce apoptosis and inhibit migration[J]. Chinese Science Bulletin科学通报,2017,62(4):270-278.
APA	Li, Jingling,陈春英,Cao, Jianping,Zhang, Leshuai,Guo, Zhengqing,&Chen, Chunying.(2017).Mor-platin a new platinum complex that can induce apoptosis and inhibit migration.Chinese Science Bulletin科学通报,62(4),270-278.
MLA	Li, Jingling,et al."Mor-platin a new platinum complex that can induce apoptosis and inhibit migration".Chinese Science Bulletin科学通报 62.4(2017):270-278.