Characterization of Sexual Trait Development in cyp17a1-Deficient Zebrafish

doi:10.1210/en.2018-00551

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Characterization of Sexual Trait Development in cyp17a1-Deficient Zebrafish
	Zhai, Gang 1; Shu, Tingting 1,2; Xia, Yuguo 3; Lu, Yao 1,2; Shang, Guohui 1,2; Jin, Xia 1; He, Jiangyan 1; Nie, Pin 1; Yin, Zhan 1
刊名	ENDOCRINOLOGY
	2018-10-01
卷号	159 期号:10 页码:3549-3562
ISSN号	0013-7227
DOI	10.1210/en.2018-00551
通讯作者	Yin, Zhan(zyin@ihb.ac.cn)
英文摘要	Cytochrome P450 (Cyp) 17A1 has both 17 alpha-hydroxylase and 17,20-lyase activities, which are involved in the steroidogenic pathway that produces androgens and estrogens. Previously, a phenotype of all-male cyp17a1-deficient zebrafish generated by transcription activatorlike effector nuclease has been reported. In the current study, the mechanisms relating to Cyp17a1 that are involved in the development of sexual traits, especially gonadal differentiation and testicular development, were characterized. We found that the cyp17a1-deficient fish at 3 months postfertilization (mpf) were all fertile males with normal testis and spermatogenesis but compromised male-typical mating behaviors and secondary sex characters (SSCs), including breeding tubercles, body pigmentation, and anal fin coloration. These results demonstrate that spermatogenesis and testicular development are not as susceptible to androgen deficiency compared with the formation of male-typical SSCs and mating behaviors in zebrafish. The differentiation of the juvenile ovary into the mature ovary failed during the critical sexual differentiation stage. This all-male phenotype of the cyp17a1-deficient fish could be restored with testosterone or estradiol treatment. For testicular development in cyp17a1-deficient fish, a gradually increasing number of spermatozoa and testis hypertrophy from 3 to 6 mpf were observed, accompanied by constitutively upregulated pituitary gonadotropin FSH subunit beta (fsh beta). The hypertrophic testis and enhanced spermatogenesis in the cyp17a1-deficient fish at 6 mpf could be effectively rescued by fshb depletion. These results confirm that adequate estrogen is essential for maintaining ovarian differentiation, and they provide new insight into the role of FSH beta in male testicular development and spermatogenesis.
资助项目	National Natural Science Foundation of China[31530077] ; National Natural Science Foundation of China[31501857] ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program)[2014CB138602] ; Pilot Program A Project from the Chinese Academy of Sciences[XDA08010405]
WOS关键词	FOLLICLE-STIMULATING-HORMONE ; ANTI-MULLERIAN-HORMONE ; GENDER-ROLE BEHAVIOR ; TARGETED DISRUPTION ; DANIO-RERIO ; OVARIAN-DIFFERENTIATION ; AROMATASE INHIBITOR ; ANDROGEN RECEPTOR ; ORYZIAS-LATIPES ; ADULT ZEBRAFISH
WOS研究方向	Endocrinology & Metabolism
语种	英语
出版者	ENDOCRINE SOC
WOS记录号	WOS:000446428300008
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/29251]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
通讯作者	Yin, Zhan
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China 3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Zhai, Gang,Shu, Tingting,Xia, Yuguo,et al. Characterization of Sexual Trait Development in cyp17a1-Deficient Zebrafish[J]. ENDOCRINOLOGY,2018,159(10):3549-3562.
APA	Zhai, Gang.,Shu, Tingting.,Xia, Yuguo.,Lu, Yao.,Shang, Guohui.,...&Yin, Zhan.(2018).Characterization of Sexual Trait Development in cyp17a1-Deficient Zebrafish.ENDOCRINOLOGY,159(10),3549-3562.
MLA	Zhai, Gang,et al."Characterization of Sexual Trait Development in cyp17a1-Deficient Zebrafish".ENDOCRINOLOGY 159.10(2018):3549-3562.