ScFv-conjugated superparamagnetic iron oxide nanoparticles for MRI-based diagnosis in transgenic mouse models of Parkinson's and Huntington's diseases

doi:10.1016/j.brainres.2018.11.034

	ScFv-conjugated superparamagnetic iron oxide nanoparticles for MRI-based diagnosis in transgenic mouse models of Parkinson's and Huntington's diseases
	Liu, Xiao-ge 1,2; Lu, Shuai 1; Liu, Dong-qun 1,2; Zhang, Lun 1,2; Zhang, Ling-xiao 1,2; Yu, Xiao-lin 1; Liu, Rui-tian 1
刊名	BRAIN RESEARCH
	2019-03-15
卷号	1707 页码:141-153
关键词	Magnetic resonance imaging Diagnosis Parkinson's disease Huntington's disease Amyloid oligomer ScFv antibody
ISSN号	0006-8993
DOI	10.1016/j.brainres.2018.11.034
英文摘要	It is widely accepted that amyloid oligomers are the most toxic species to initiate the pathologic processes of Parkinson's disease (PD) and Huntingdon's disease (HD). But there is no definitive diagnosis for PD and HD at their early stages. Here, we conjugated an amyloid oligomer-specific scFv antibody (W20) to PEGylated superparamagnetic iron oxide nanoparticles (SPIONs) and detected the properties of the SPIONs conjugated with W20. The results showed that W20-SPIONs, with the size of around 11.8 nm in diameter, were stable and nontoxic, and had enough relaxation capacity to be used as an MRI contrast agent. When applied to the transgenic mouse models of PD and HD, W20-SPIONs crossed the blood-brain barrier and specifically bound to the oligomer area to give MRI signal, distinguishing PD and HD from healthy controls. These results indicated that W20-SPIONs had potential in early-stage diagnosis for PD and HD and also opened up a new strategy for evaluating the efficacy of new drugs.
资助项目	National Science and Technology Major Projects of New Drugs[2018ZX09301032] ; National Science and Technology Major Projects of New Drugs[2018ZX09733001-001-008] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA12040215]
WOS关键词	ANTIBODIES SPECIFICALLY RECOGNIZE ; MAGNETIC NANOPARTICLES ; MOLECULAR-MECHANISMS ; BRAIN ; OLIGOMERS ; DELIVERY ; LESIONS ; DESIGN ; MICE
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000459842000016
资助机构	National Science and Technology Major Projects of New Drugs ; Strategic Priority Research Program of Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/28289]
专题	中国科学院过程工程研究所
通讯作者	Yu, Xiao-lin; Liu, Rui-tian
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiao-ge,Lu, Shuai,Liu, Dong-qun,et al. ScFv-conjugated superparamagnetic iron oxide nanoparticles for MRI-based diagnosis in transgenic mouse models of Parkinson's and Huntington's diseases[J]. BRAIN RESEARCH,2019,1707:141-153.
APA	Liu, Xiao-ge.,Lu, Shuai.,Liu, Dong-qun.,Zhang, Lun.,Zhang, Ling-xiao.,...&Liu, Rui-tian.(2019).ScFv-conjugated superparamagnetic iron oxide nanoparticles for MRI-based diagnosis in transgenic mouse models of Parkinson's and Huntington's diseases.BRAIN RESEARCH,1707,141-153.
MLA	Liu, Xiao-ge,et al."ScFv-conjugated superparamagnetic iron oxide nanoparticles for MRI-based diagnosis in transgenic mouse models of Parkinson's and Huntington's diseases".BRAIN RESEARCH 1707(2019):141-153.