Ultrasensitive On-Site Detection of Biological Active Ricin in Complex Food Matrices Based on Immunomagnetic Enrichment and Fluorescence Switch-On Nanoprobe | |
Sung, Jiefang1; Zhang, Xueyong3; Li, Ting4; Xie, Jijia5; Shao, Bing1,2; Xue, Dingshuai6; Tang, Xu6; Li, Hui1; Liu, Yanhong6 | |
刊名 | ANALYTICAL CHEMISTRY |
2019-05-21 | |
卷号 | 91期号:10页码:6454-6461 |
ISSN号 | 0003-2700 |
DOI | 10.1021/acs.analchem.8b04458 |
英文摘要 | Ricin is a highly toxic protein largely existing in castor beans, which could be used as a warfare agent due to its unique properties. As a deadenylase, inactivation of ricin means a loss of its toxic threat. Therefore, developing simple, accurate, and sensitive on-site detection of biologically active ricin in wide types of complex matrices is most valuable. Here, antifouling polymer brush modified magnetic beads were prepared first and post modified with ricin monoclonal antibody (the MB@P(C-H)-mAb(ricin)) to efficiently capture ricin from various foods and biological matrices. Active ricin obtained in this manner were sequentially determined by a new designed AuNP/QDs nanoassembly. In this double strand oligodeoxynucleotides (dsODN) linked core-satellite nanoprobe, the fluorescence of satellite QDs was extensively quenched by AuNPs due to the dipole-metal interaction. Active ricin can react with its specific depurination substrates which had been inserted in the dsODN linkers. This reaction would trigger the separation of QDs from Au cores by cutting multiple adenines, and then result in the restoration of QDs fluorescence. By coupling with the magnetic enrichment, this AuNP/QDs nanoprobe provided a qualitative result for active ricin in the range from 10.0 to 100.0 ng mL(-1) with the limit of detection as low as 7.46 ng mL(-1). Compared with previously proposed methods, this on-site detection strategy offered an easy to handle on-site test for trace amounts of active ricin in a wide range of complex matrices. |
资助项目 | National Natural Science Foundation of China[21677019] ; National Natural Science Foundation of China[41403021] ; National Natural Science Foundation of China[U1736201] ; Capital Health Research and Development of Special[2018-4-1014] ; key research and development program of Beijing[D171100008317001] |
WOS关键词 | GOLD NANOPARTICLES ; REAL-TIME ; IDENTIFICATION ; QUANTIFICATION ; DIGESTION ; ASSAY |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000469304300010 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; Capital Health Research and Development of Special ; Capital Health Research and Development of Special ; key research and development program of Beijing ; key research and development program of Beijing ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Capital Health Research and Development of Special ; Capital Health Research and Development of Special ; key research and development program of Beijing ; key research and development program of Beijing ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Capital Health Research and Development of Special ; Capital Health Research and Development of Special ; key research and development program of Beijing ; key research and development program of Beijing ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Capital Health Research and Development of Special ; Capital Health Research and Development of Special ; key research and development program of Beijing ; key research and development program of Beijing |
内容类型 | 期刊论文 |
源URL | [http://ir.iggcas.ac.cn/handle/132A11/92281] |
专题 | 地质与地球物理研究所_岩石圈演化国家重点实验室 |
通讯作者 | Shao, Bing |
作者单位 | 1.Beijing Ctr Dis Prevent & Control, Beijing Key Lab Diagnost & Traceabil Technol Food, Beijing 100013, Peoples R China 2.China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100193, Peoples R China 3.Peoples Publ Secur Univ China, Sch Police Law Enforcement Abil Training, Beijing 100038, Peoples R China 4.Capital Med Univ, Sch Publ Hlth, Beijing 100069, Peoples R China 5.UCL, Dept Chem Engn, Torrington Pl, London WC1E 7JE, England 6.Chinese Acad Sci, Inst Geol & Geophys, State Key Lab Lithospher Evolut, Beijing 100029, Peoples R China |
推荐引用方式 GB/T 7714 | Sung, Jiefang,Zhang, Xueyong,Li, Ting,et al. Ultrasensitive On-Site Detection of Biological Active Ricin in Complex Food Matrices Based on Immunomagnetic Enrichment and Fluorescence Switch-On Nanoprobe[J]. ANALYTICAL CHEMISTRY,2019,91(10):6454-6461. |
APA | Sung, Jiefang.,Zhang, Xueyong.,Li, Ting.,Xie, Jijia.,Shao, Bing.,...&Liu, Yanhong.(2019).Ultrasensitive On-Site Detection of Biological Active Ricin in Complex Food Matrices Based on Immunomagnetic Enrichment and Fluorescence Switch-On Nanoprobe.ANALYTICAL CHEMISTRY,91(10),6454-6461. |
MLA | Sung, Jiefang,et al."Ultrasensitive On-Site Detection of Biological Active Ricin in Complex Food Matrices Based on Immunomagnetic Enrichment and Fluorescence Switch-On Nanoprobe".ANALYTICAL CHEMISTRY 91.10(2019):6454-6461. |
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