Simultaneous metabolomics and lipidomics analysis based on novel heart-cutting two-dimensional liquid chromatography-mass spectrometry

doi:10.1016/j.aca.2017.03.004

	Simultaneous metabolomics and lipidomics analysis based on novel heart-cutting two-dimensional liquid chromatography-mass spectrometry
	Wang, Shuangyuan 1,2; Zhou, Lina 1; Wang, Zhichao 1,2; Shi, Xianzhe 1; Xu, Guowang 1
刊名	ANALYTICA CHIMICA ACTA
	2017-05-08
卷号	966 页码:34-40
关键词	Metabolomics Lipidomics Metabolite Coverage Two-dimensional Liquid Chromatography Heart-cutting
ISSN号	0003-2670
DOI	10.1016/j.aca.2017.03.004
文献子类	Article
英文摘要	Increasing metabolite coverage by combining data from different platforms or methods can improve understanding of related metabolic mechanisms and the identification of biomarkers. However, no one method can obtain metabolomic and lipidomic information in a single analysis. In this work, aiming at collecting comprehensive information on metabolome and lipidome in a single analytical run, we developed an on-line heart-cutting two-dimensional liquid chromatography-mass spectrometry (2D-LCMS) method. Complex metabolites from biological samples are divided into two fractions by using a precolumn. The first fraction is directly transferred and subjected to metabolomics analysis. Most lipids are retained on the precolumn until the mobile phases for lipidomics flow through; then they are subjected to lipidomics analysis. Up to 447 and 289 metabolites in plasma, including amino acids, carnitines, bile acids, free fatty acids, lyso-phospholipids, phospholipids, sphingomyelins etc. were identified within 30 min in the positive mode and negative mode, respectively. A comparison of the newly developed method with the conventional metabolomic and lipidomic approaches showed that approximately 99% features obtained by the two conventional methods can be covered with this 2D-LC method. Analytical characteristics evaluation showed the method had a wide linearity range, high sensitivity, satisfactory recovery and repeatability. These results demonstrate that this method is reliable, stable and well qualified in metabolomics analysis, particularly for large-scale metabolomics studies with small amount of samples. (C) 2017 Elsevier B.V. All rights reserved.
WOS关键词	TARGETED METABOLOMICS ; REVERSED-PHASE ; UPLC-MS ; COVERAGE ; SEPARATION ; PLASMA ; IDENTIFICATION ; METABOLISM ; DISEASE ; CANCER
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000398435500003
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169312]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Shi, Xianzhe; Xu, Guowang
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Shuangyuan,Zhou, Lina,Wang, Zhichao,et al. Simultaneous metabolomics and lipidomics analysis based on novel heart-cutting two-dimensional liquid chromatography-mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2017,966:34-40.
APA	Wang, Shuangyuan,Zhou, Lina,Wang, Zhichao,Shi, Xianzhe,&Xu, Guowang.(2017).Simultaneous metabolomics and lipidomics analysis based on novel heart-cutting two-dimensional liquid chromatography-mass spectrometry.ANALYTICA CHIMICA ACTA,966,34-40.
MLA	Wang, Shuangyuan,et al."Simultaneous metabolomics and lipidomics analysis based on novel heart-cutting two-dimensional liquid chromatography-mass spectrometry".ANALYTICA CHIMICA ACTA 966(2017):34-40.