Boronic acid-functionalized iron oxide magnetic nanoparticles via distillation-precipitation polymerization and thiol-yne click chemistry for the enrichment of glycoproteins

doi:10.1039/c8nj01711e

	Boronic acid-functionalized iron oxide magnetic nanoparticles via distillation-precipitation polymerization and thiol-yne click chemistry for the enrichment of glycoproteins
	Chen, Langxing 2,5; Bi, Changfen 1,4; Zhang, Sitong 5; Li, Yiliang 1,4; He, Xiwen 5; Zhang, Yukui 3,5
刊名	NEW JOURNAL OF CHEMISTRY
	2018-11-07
卷号	42 期号:21 页码:17331-17338
ISSN号	1144-0546
DOI	10.1039/c8nj01711e
通讯作者	Li, Yiliang() ; Chen, Langxing(lxchen@nankai.edu.cn)
英文摘要	In this work, a novel strategy is developed for the fabrication of phenylboronic acid functionalized iron oxide magnetic nanoparticles (MNPs) via distillation-precipitation polymerization and thiol-yne click chemistry. Firstly, silica was coated on the Fe3O4 MNPs using a sol-gel method to obtain Fe3O4@SiO2. Subsequently, the vinyl groups were grated onto the silica-modified Fe3O4 surface using 3-methacryloyloxypropyltrimethoxysilane. Next, alkynyl groups as the clickable sites were modified on the MNPs to obtain the product Fe3O4@pPMA via distillation-precipitation polymerization of the vinyl end groups with propargyl methacrylate, ethylene glycol dimethacrylate. Finally, phenylboronic acid was grafted on the surface of Fe3O4@pPMA via thiol-yne click chemistry. The morphology, structure, and composition of all the synthesized phenylboronic acid functionalized Fe3O4 MNPs (Fe3O4@pPMA-MPBA) were characterized by transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray powder diffraction (XRD), vibrating sample magnetometry (VSM), X-ray photoelectron spectroscopy (XPS) and thermogravimetric analysis (TGA). Three glycoproteins ovalbumin, transferrin, and horseradish peroxidase and two non-glycoproteins lysozyme and horse heart cytochrome are the chosen target proteins and their adsorption performance on Fe3O4@pPMA-MPBA is investigated. The Fe3O4@pPMA-MPBA MNPs exhibited outstanding advantages towards glycoproteins including high adsorption capacity, excellent specificity, repeatability and good enrichment recovery. Furthermore, the Fe3O4@pPMA-MPBA MNPs could efficiently enrich glycoproteins from real egg white samples. This study provides a feasible strategy for the surface functionalization of boronate affinity nanomaterials for the selective isolation and enrichment of glycoproteins.
资助项目	National Natural Science Foundation of China[21475067] ; Natural Science Foundation of Tianjin City[15JCYBJC20600] ; Natural Science Foundation of Tianjin City[18JCQNJC09500] ; CAMS Innovation Fund for Medical Sciences (CIFMS)[2017-I2M-3-019] ; CAMS Innovation Fund for Medical Sciences (CIFMS)[2016-I2M-3-022]
WOS关键词	TRANSFER RADICAL POLYMERIZATION ; SELECTIVE ENRICHMENT ; TRACE GLYCOPROTEINS ; EFFICIENT APPROACH ; GLYCOSYLATION ; CANCER ; RECOGNITION ; METASTASIS ; COMPOSITES ; CAPTURE
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000448568100017
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Tianjin City ; Natural Science Foundation of Tianjin City ; CAMS Innovation Fund for Medical Sciences (CIFMS) ; CAMS Innovation Fund for Medical Sciences (CIFMS) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Tianjin City ; Natural Science Foundation of Tianjin City ; CAMS Innovation Fund for Medical Sciences (CIFMS) ; CAMS Innovation Fund for Medical Sciences (CIFMS) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Tianjin City ; Natural Science Foundation of Tianjin City ; CAMS Innovation Fund for Medical Sciences (CIFMS) ; CAMS Innovation Fund for Medical Sciences (CIFMS) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Tianjin City ; Natural Science Foundation of Tianjin City ; CAMS Innovation Fund for Medical Sciences (CIFMS) ; CAMS Innovation Fund for Medical Sciences (CIFMS)
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166778]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Chen, Langxing; Li, Yiliang
作者单位	1.Chinese Acad Med Sci, Tianjin 300192, Peoples R China 2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 4.Peking Union Med Coll, Inst Radiat Med, Tianjin Key Lab Radiat Med & Mol Nucl Med, Tianjin 300192, Peoples R China 5.Nankai Univ, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Res Ctr Analyt Sci,Coll Chem, Tianjin 300071, Peoples R China
推荐引用方式 GB/T 7714	Chen, Langxing,Bi, Changfen,Zhang, Sitong,et al. Boronic acid-functionalized iron oxide magnetic nanoparticles via distillation-precipitation polymerization and thiol-yne click chemistry for the enrichment of glycoproteins[J]. NEW JOURNAL OF CHEMISTRY,2018,42(21):17331-17338.
APA	Chen, Langxing,Bi, Changfen,Zhang, Sitong,Li, Yiliang,He, Xiwen,&Zhang, Yukui.(2018).Boronic acid-functionalized iron oxide magnetic nanoparticles via distillation-precipitation polymerization and thiol-yne click chemistry for the enrichment of glycoproteins.NEW JOURNAL OF CHEMISTRY,42(21),17331-17338.
MLA	Chen, Langxing,et al."Boronic acid-functionalized iron oxide magnetic nanoparticles via distillation-precipitation polymerization and thiol-yne click chemistry for the enrichment of glycoproteins".NEW JOURNAL OF CHEMISTRY 42.21(2018):17331-17338.