Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts

doi:10.1007/s00299-018-2317-2

	Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts
	Badiali, Camilla 3; De Angelis, Giulia 3; Simonetti, Giovanna 1; Brasili, Elisa 2,3; Tobaruela, Eric de Castro 2; Purgatto, Eduardo 2; Yin, Heng 4; Valletta, Alessio 3; Pasqua, Gabriella 3
刊名	PLANT CELL REPORTS
	2018-11-01
卷号	37 期号:11 页码:1471-1484
关键词	Hypericum perforatum Root cultures Chitooligosaccharides Xanthones Volatile organic compounds
ISSN号	0721-7714
DOI	10.1007/s00299-018-2317-2
通讯作者	Valletta, Alessio(alessio.valletta@uniroma1.it)
英文摘要	Key messageWater-soluble chitosan oligosaccharides (COS) affect xanthone and volatile organic compound content, as well as antifungal activity against human pathogenic fungi of extracts obtained from Hypericum perforatum root cultures.AbstractSeveral studies have demonstrated the elicitor power of chitosan on xanthone biosynthesis in root cultures of H. perforatum. One of the major limitations to the use of chitosan, both for basic and applied research, is the need to use acidified water for solubilization. To overcome this problem, the elicitor effect of water-soluble COS on the biosynthesis of both xanthones and volatile organic compounds (VOCs) was evaluated in the present study. The analysis of xanthones and VOCs was performed by HPLC and GC-MS headspace analysis. The obtained results showed that COS are very effective in enhancing xanthone biosynthesis. With 400mgL(-1) COS, a xanthone content of about 30mgg(-1) DW was obtained. The antifungal activity of extracts obtained with 400mgL(-1) COS was the highest, with MIC50 of 32 mu gmL(-1) against Candida albicans and 32-64 mu g mL(-1) against dermatophytes, depending on the microorganism. Histochemical investigations suggested the accumulation of isoprenoids in the secretory ducts of H. perforatum roots. The presence of monoterpenes and sesquiterpenes was confirmed by the headspace analysis. Other volatile hydrocarbons have been identified. The biosynthesis of most VOCs showed significant changes in response to COS, suggesting their involvement in plant-fungus interactions.
资助项目	Sapienza Universita di Roma[C26A15MLP9] ; Sapienza Universita di Roma[AR11715C81A4ABDF]
WOS关键词	ESSENTIAL OIL COMPOSITION ; CELL-SUSPENSION CULTURES ; HAIRY ROOTS ; L. ; DEATH ; ACCUMULATION ; ELICITORS ; BIOMASS ; GROWTH ; OLIGOCHITOSAN
WOS研究方向	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000451470600002
资助机构	Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma ; Sapienza Universita di Roma
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166598]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Valletta, Alessio
作者单位	1.Sapienza Univ Rome, Dept Publ Hlth & Infect Dis, Piazzale Aldo Moro 5, I-00185 Rome, Italy 2.Univ Sao Paulo, Dept Food Sci & Expt Nutr, FORC Food Res Ctr, Av Prof Lineu Prestes 580, BR-05508000 Sao Paulo, Brazil 3.Sapienza Univ Rome, Dept Environm Biol, Piazzale Aldo Moro 5, I-00185 Rome, Italy 4.Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Badiali, Camilla,De Angelis, Giulia,Simonetti, Giovanna,et al. Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts[J]. PLANT CELL REPORTS,2018,37(11):1471-1484.
APA	Badiali, Camilla.,De Angelis, Giulia.,Simonetti, Giovanna.,Brasili, Elisa.,Tobaruela, Eric de Castro.,...&Pasqua, Gabriella.(2018).Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts.PLANT CELL REPORTS,37(11),1471-1484.
MLA	Badiali, Camilla,et al."Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts".PLANT CELL REPORTS 37.11(2018):1471-1484.