Integrated Metabolomics and Lipidomics Analyses Reveal Metabolic Reprogramming in Human Glioma with IDH1 Mutation

doi:10.1021/acs.jproteome.8b00663

	Integrated Metabolomics and Lipidomics Analyses Reveal Metabolic Reprogramming in Human Glioma with IDH1 Mutation
	Xu, Guowang 1; Zhou, Lina 1; Wang, Zhichao 1,2; Hu, Chunxiu 1; Zhang, Chaoqi 3; Kovatcheva-Datchary, Petia 1; Yu, Di 1,2; Liu, Shasha 3; Ren, Feifei 3; Wang, Xiaolin 1
刊名	JOURNAL OF PROTEOME RESEARCH
	2019-03-01
卷号	18 期号:3 页码:960-969
关键词	glioma IDH1 metabolomics lipidomics metabolic reprogramming
ISSN号	1535-3893
DOI	10.1021/acs.jproteome.8b00663
通讯作者	Zhang, Yi(yizhang@zzu.edu.cn) ; Xu, Guowang()
英文摘要	Mutations in isocitrate dehydrogenase (IDH) 1 are high-frequency events in low-grade glioma and secondary glioblastoma, and IDH1 mutant gliomas are vulnerable to interventions. Metabolic reprogramming is a hallmark of cancer. In this study, comprehensive metabolism investigation of clinical IDH1 mutant glioma specimens was performed to explore its specific metabolic reprogramming in real micro environment. Massive metabolic alterations from glycolysis to lipid metabolism were identified in the IDH1 mutant glioma tissue when compared to IDH1 wild-type glioma. Of note, tricarboxylic acid (TCA) cycle intermediates were in similar levels in both groups, with more pyruvate found entering the TCA cycle in IDH1 mutant glioma. The pool of fatty acyl chains was also reduced, displayed as decreased triglycerides and sphingolipids, although membrane phosphatidyl lipids were not changed. The lower fatty acyl pool may be mediated by the lower protein expression levels of long-chain acyl-CoA synthetase 1 (ACSL1), ACSL4, and very long-chain acyl-CoA synthetase 3 (ACSVL3) in IDH1 mutant glioma. Lower ACSL1 was further found to contribute to the better survival of IDH1 mutant glioma patients based on the The Cancer Genome Atlas (TCGA) RNA sequencing data. Our research provides valuable insights into the tissue metabolism of human IDH1 mutant glioma and unravels new lipid-related targets.
资助项目	National Natural Science Foundation of China[81472374] ; National Natural Science Foundation of China[21505132] ; National Natural Science Foundation of China[21435006] ; [2017YFC0906900]
WOS关键词	ISOCITRATE DEHYDROGENASE 1 ; OXIDATIVE STRESS ; R132H MUTATION ; CANCER ; PROFILES ; GROWTH ; CELLS
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000460491800014
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/165894]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Xu, Guowang; Zhang, Yi
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Zhengzhou Univ, Affiliated Hosp 1, Biotherapy Ctr, Zhengzhou 450052, Henan, Peoples R China
推荐引用方式 GB/T 7714	Xu, Guowang,Zhou, Lina,Wang, Zhichao,et al. Integrated Metabolomics and Lipidomics Analyses Reveal Metabolic Reprogramming in Human Glioma with IDH1 Mutation[J]. JOURNAL OF PROTEOME RESEARCH,2019,18(3):960-969.
APA	Xu, Guowang.,Zhou, Lina.,Wang, Zhichao.,Hu, Chunxiu.,Zhang, Chaoqi.,...&Zhang, Yi.(2019).Integrated Metabolomics and Lipidomics Analyses Reveal Metabolic Reprogramming in Human Glioma with IDH1 Mutation.JOURNAL OF PROTEOME RESEARCH,18(3),960-969.
MLA	Xu, Guowang,et al."Integrated Metabolomics and Lipidomics Analyses Reveal Metabolic Reprogramming in Human Glioma with IDH1 Mutation".JOURNAL OF PROTEOME RESEARCH 18.3(2019):960-969.