Multiplexed profiling of single-cell extracellular vesicles secretion

doi:10.1073/pnas.1814348116

	Multiplexed profiling of single-cell extracellular vesicles secretion
	Su, Haoran 2,3; Ji, Yahui 2,3; Qi, Dongyuan 4; Li, Linmei 3; Li, Xiaojie 2; Luo, Yong 5; Sun, Bo 1; Zhang, Fuyin 1; Lin, Bingcheng 3; Liu, Tingjiao 2
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2019-03-26
卷号	116 期号:13 页码:5979-5984
关键词	single-cell analysis extracellular vesicle cellular heterogeneity antibody barcodes
ISSN号	0027-8424
DOI	10.1073/pnas.1814348116
通讯作者	Liu, Tingjiao(tingjiao@dmu.edu.cn) ; Lu, Yao(luyao@dicp.ac.cn)
英文摘要	Extracellular vesicles (EVs) are important intercellular mediators regulating health and diseases. Conventional methods for EV surface marker profiling, which was based on population measurements, masked the cell-to-cell heterogeneity in the quantity and phenotypes of EV secretion. Herein, by using spatially patterned antibody barcodes, we realized multiplexed profiling of single-cell EV secretion from more than 1,000 single cells simultaneously. Applying this platform to profile human oral squamous cell carcinoma (OSCC) cell lines led to a deep understanding of previously undifferentiated single-cell heterogeneity underlying EV secretion. Notably, we observed that the decrement of certain EV phenotypes (e.g., (CD63+) V) was associated with the invasive feature of both OSCC cell lines and primary OSCC cells. We also realized multiplexed detection of EV secretion and cytokines secretion simultaneously from the same single cells to investigate the multidimensional spectrum of cellular communications, from which we resolved tiered functional subgroups with distinct secretion profiles by visualized clustering and principal component analysis. In particular, we found that different cell subgroups dominated EV secretion and cytokine secretion. The technology introduced here enables a comprehensive evaluation of EV secretion heterogeneity at single-cell level, which may become an indispensable tool to complement current single-cell analysis and EV research.
资助项目	National Natural Science Foundation of China[21874133] ; National Natural Science Foundation of China[21605143] ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant[2018217] ; Dalian Institute of Chemical Physics Grant[SZ201601]
WOS关键词	CD63 TETRASPANIN ; EXOSOME SECRETION ; HETEROGENEITY ; REVEALS ; EXPRESSION ; MICROVESICLES ; CARCINOMA ; CYTOKINE ; RELEASE
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATL ACAD SCIENCES
WOS记录号	WOS:000462382800029
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/165702]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Liu, Tingjiao; Lu, Yao
作者单位	1.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China 2.Dalian Med Univ, Coll Stomatol, Dalian 116044, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 4.Dalian Med Univ, Affiliated Hosp 1, Dalian 116011, Peoples R China 5.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China
推荐引用方式 GB/T 7714	Su, Haoran,Ji, Yahui,Qi, Dongyuan,et al. Multiplexed profiling of single-cell extracellular vesicles secretion[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(13):5979-5984.
APA	Su, Haoran.,Ji, Yahui.,Qi, Dongyuan.,Li, Linmei.,Li, Xiaojie.,...&Lu, Yao.(2019).Multiplexed profiling of single-cell extracellular vesicles secretion.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(13),5979-5984.
MLA	Su, Haoran,et al."Multiplexed profiling of single-cell extracellular vesicles secretion".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.13(2019):5979-5984.