QQ客服 官方微博 反馈留言

	Cflec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate c-type lectin
	Yang, Jialong1,3; Huang, Mengmeng1,3; Zhang, Huan1; Wang, Lingling1; Wang, Hao1; Wang, Leilei1,3; Qiu, Limei1; Song, Linsheng1,2
刊名	Scientific reports
	2015-05-15
卷号	5页码:15
ISSN号	2045-2322
DOI	10.1038/srep10068
通讯作者	Wang, leilei(wanglingling@qdio.ac.cn)
英文摘要	A c-type lectin (cflec-3) from chlamys farreri with three carbohydrate-recognition domains (crds) was selected to dissect the possible mechanisms of pamp binding and functional differentiation of invertebrate lectins. cflec-3 distributed broadly, and its mrna expression in hemocytes increased significantly after stimulations with lps, pgn or beta-glucan, but not poly(i: c). the recombinant cflec-3 (rcflec-3) could bind pamps and several microbes. rcflec-3 mediated hemocytes phagocytosis against escherichia coli and encapsulation towards agarose beads. obvious functional differentiation occurred among the three crds, as crd1 exhibited higher activity to bind pamps, while crd2/3 were expert in promoting hemocyte mediated opsonisation. the tertiary structural differences were suspected to be associated with such functional differentiation. pamp binding abilities of cflec-3 were determined by ca2+-binding site 2 motif. when pro in this motif of each crd was mutated into ser, their pamp binding abilities were deprived absolutely. rcrd2 acquired mannan binding capability when its epd was replaced by epn, but lost when epn in rcrd3 was changed into epd. the pro in ca2+-binding site 2 was indispensable for pamps binding, while asn was determinant for specific binding to mannan. it shed new insight into pamps binding mechanism of invertebrate c-type lectins and their functional differentiation.
WOS关键词	CHLAMYS-FARRERI ; MANDUCA-SEXTA ; CELLULAR ENCAPSULATION ; ARGOPECTEN-IRRADIANS ; PATHOGEN RECOGNITION ; IMMUNE-RESPONSES ; DC-SIGN ; RECEPTORS ; PROTEIN ; BACTERIA
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000355247500002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376627
专题	中国科学院大学
通讯作者	Wang, Leilei
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Dalian Ocean Univ, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yang, Jialong,Huang, Mengmeng,Zhang, Huan,et al. Cflec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate c-type lectin[J]. Scientific reports,2015,5:15.
APA	Yang, Jialong.,Huang, Mengmeng.,Zhang, Huan.,Wang, Lingling.,Wang, Hao.,...&Song, Linsheng.(2015).Cflec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate c-type lectin.Scientific reports,5,15.
MLA	Yang, Jialong,et al."Cflec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate c-type lectin".Scientific reports 5(2015):15.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。