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	Quantitative analysis of an engineered co2-fixing escherichia coli reveals great potential of heterotrophic co2 fixation
	Gong, Fuyu1,2; Liu, Guoxia1; Zhai, Xiaoyun1,2; Zhou, Jie1; Cai, Zhen1; Li, Yin1
刊名	Biotechnology for biofuels
	2015-06-18
卷号	8页码:10
关键词	Carbon fixation Co2-fixation rate Heterotrophic microbe Carbonic anhydrase Rubisco
ISSN号	1754-6834
DOI	10.1186/s13068-015-0268-1
通讯作者	Cai, zhen(caiz@im.ac.cn)
英文摘要	Background: production of fuels from the abundant and wasteful co2 is a promising approach to reduce carbon emission and consumption of fossil fuels. autotrophic microbes naturally assimilate co2 using energy from light, hydrogen, and/or sulfur. however, their slow growth rates call for investigation of the possibility of heterotrophic co2 fixation. although preliminary research has suggested that co2 fixation in heterotrophic microbes is feasible after incorporation of a co2-fixing bypass into the central carbon metabolic pathway, it remains unclear how much and how efficient that co2 can be fixed by a heterotrophic microbe. results: a simple metabolic flux index was developed to indicate the relative strength of the co2-fixation flux. when two sequential enzymes of the cyanobacterial calvin cycle were incorporated into an e. coli strain, the flux of the co2-fixing bypass pathway accounts for 13 % of that of the central carbon metabolic pathway. the value was increased to 17 % when the carbonic anhydrase involved in the cyanobacterial carbon concentrating mechanism was introduced, indicating that low intracellular co2 concentration is one limiting factor for co2 fixation in e. coli. the engineered co2-fixing e. coli with carbonic anhydrase was able to fix co2 at a rate of 19.6 mg co2 l-1 h(-1) or the specific rate of 22.5 mg co2 g dcw-1 h(-1). this co2 fixation rate is comparable with the reported rates of 14 autotrophic cyanobacteria and algae (10.5-147.0 mg co2 l-1 h(-1) or the specific rates of 3.5-23.7 mg co2 g dcw-1 h(-1)). conclusions: the ability of co2 fixation was created and improved in e. coli by incorporating partial cyanobacterial calvin cycle and carbon concentrating mechanism, respectively. quantitative analysis revealed that the co2-fixation rate of this strain is comparable with that of the autotrophic cyanobacteria and algae, demonstrating great potential of heterotrophic co2 fixation.
WOS关键词	CARBON-DIOXIDE FIXATION ; CHROMATOGRAPHY-MASS SPECTROMETRY ; IMPROVING PHOTOSYNTHESIS ; BIOMASS PRODUCTION ; C-13/C-12 RATIO ; ATMOSPHERIC CO2 ; LACTIC-ACID ; RUBISCO ; CYANOBACTERIA ; MICROALGAE
WOS研究方向	Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000356476800001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376452
专题	中国科学院大学
通讯作者	Cai, Zhen
作者单位	1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Gong, Fuyu,Liu, Guoxia,Zhai, Xiaoyun,et al. Quantitative analysis of an engineered co2-fixing escherichia coli reveals great potential of heterotrophic co2 fixation[J]. Biotechnology for biofuels,2015,8:10.
APA	Gong, Fuyu,Liu, Guoxia,Zhai, Xiaoyun,Zhou, Jie,Cai, Zhen,&Li, Yin.(2015).Quantitative analysis of an engineered co2-fixing escherichia coli reveals great potential of heterotrophic co2 fixation.Biotechnology for biofuels,8,10.
MLA	Gong, Fuyu,et al."Quantitative analysis of an engineered co2-fixing escherichia coli reveals great potential of heterotrophic co2 fixation".Biotechnology for biofuels 8(2015):10.

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