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	Hsv-2 glycoprotein gd targets the cc domain of tetherin and promotes tetherin degradation via lysosomal pathway
	Liu, Yalan1; Li, Mei1,2; Zhang, Di1,2; Zhang, Mudan1; Hu, Qinxue1,3
刊名	Virology journal
	2016-09-15
卷号	13页码:9
关键词	Hsv-2 glycoprotein d Tetherin Interaction Lysosomal degradation
ISSN号	1743-422X
DOI	10.1186/s12985-016-0610-7
通讯作者	Liu, yalan(liuyl@wh.iov.cn)
英文摘要	Background: hsv-2 is the major cause of genital herpes. we previously demonstrated that the host viral restriction factor tetherin restricts hsv-2 release and is antagonized by several hsv-2 glycoproteins. however, the mechanisms underlying hsv-2 glycoproteins mediated counteraction of tetherin remain unclear. in this study, we investigated whether tetherin restricts the cell-to-cell spread of hsv-2 and the mechanisms underlying hsv-2 gd mediated antagonism of tetherin. methods: infectious center assays were used to test whether tetherin could affect cell-to-cell spread of hsv-2. coimmunoprecipitation assays were performed to map the tetherin domains required for hsv-2 gd-mediated downregulation. immunoflurence assays were performed to detect the accumulation of tetherin in lysosomes or proteasomes. all experiments were repeated for at least three times and the data were performed statistical analysis. results: 1) tetherin restricts cell-to-cell spread of hsv-2; 2) hsv-2 gd specifically interacts with the cc domain of tetherin; 3) hsv-2 gd promotes tetherin to the lysosomal degradation pathway. conclusions: tetherin not only restricts hsv-2 release but also its cell-to-cell spread. in turn, hsv-2 gd targets the cc domain of tetherin and promotes its degradation in the lysosome. findings in this study have increased our understanding of tetherin restriction and viral countermeasures.
WOS关键词	HERPES-SIMPLEX-VIRUS ; GENITAL ULCER DISEASE ; TO-CELL SPREAD ; HIV-1 VPU ; RESTRICTION FACTOR ; TYPE-2 INFECTION ; HEPARAN-SULFATE ; DOWN-REGULATION ; RELEASE ; BST2/TETHERIN
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000383423500001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376301
专题	中国科学院大学
通讯作者	Liu, Yalan
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.St Georges Univ London, Inst Infect & Immun, London SW17 0RE, England
推荐引用方式 GB/T 7714	Liu, Yalan,Li, Mei,Zhang, Di,et al. Hsv-2 glycoprotein gd targets the cc domain of tetherin and promotes tetherin degradation via lysosomal pathway[J]. Virology journal,2016,13:9.
APA	Liu, Yalan,Li, Mei,Zhang, Di,Zhang, Mudan,&Hu, Qinxue.(2016).Hsv-2 glycoprotein gd targets the cc domain of tetherin and promotes tetherin degradation via lysosomal pathway.Virology journal,13,9.
MLA	Liu, Yalan,et al."Hsv-2 glycoprotein gd targets the cc domain of tetherin and promotes tetherin degradation via lysosomal pathway".Virology journal 13(2016):9.

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