QQ客服 官方微博 反馈留言

	The cytochemical and ultrastructural characteristics of phagocytes in the pacific oyster crassostrea gigas
	Jiang, Shuai1; Jia, Zhihao1,3; Xin, Lusheng1,3; Sun, Ying1,3; Zhang, Ran1; Wang, Weilin1,3; Wang, Lingling2; Song, Linsheng2
刊名	Fish & shellfish immunology
	2016-08-01
卷号	55页码:490-498
关键词	Crassostrea gigas Phagocyte Cytochemical staining Ultrastructural analysis Cytokine Lysosome Reactive oxygen species
ISSN号	1050-4648
DOI	10.1016/j.fsi.2016.06.024
通讯作者	Song, linsheng(lshsong@dlou.edu.cn)
英文摘要	Phagocytes have been proved to play vital roles in the innate immune response. however, the cellular characteristics of phagocytes in invertebrates, especially in molluscs, remain largely unknown. in the present study, fluorescence activated cell sorting (frcs) was employed to sort the phagocytes from the non-phagocytic haemocytes of the pacific oyster crassostrea gigas. the cytochemical staining analysis revealed that phagocytes were positive staining for alpha-naphthyl acetate esterase and myeloperoxidase, while negative staining for toluidine blue and periodic acid-schiff. the non-phagocytic haemocytes exhibited positive staining for periodic acid-schiff, weak positive staining for toluidine blue, but negative staining for alpha-naphthyl acetate esterase and myeloperoxidase. in addition, phagocytes exhibited ultra structural cellular features similar to those of macrophages, with large cell diameter, rough cell membrane and extended pseudopodia revealed by the scanning electron microscopy, while the non-phagocytic haemocytes exhibited small cell diameter, smooth cell surface and round spherical shape. transmission electron microscopy further demonstrated that phagocytes were abundant of cytoplasmic bodies and mitochondria, while non-phagocytic haemocytes were characterized as the comparatively large cell nucleus with contorted and condensed heterochromatin adherent to the nuclear envelope. moreover, compared with non-phagocytic haemocytes, phagocytes exhibited significantly higher levels of intracellular cytokines, including tumor necrosis factor, interferon-like protein and interleukin-17, and significantly higher abundance of lysosome and reactive oxygen species, which were of great importance to the activation of immune response and pathogen clearance. taken together, these findings revealed the different cytochemical and ultrastructural features between phagocytes and non-phagocytic haemocytes in c. gigas, which would provide an important clue to investigate the mechanism of phagocytosis underlying the innate immune response. (c) 2016 elsevier ltd. all rights reserved.
WOS关键词	INNATE IMMUNITY ; DEPENDENT PHAGOCYTOSIS ; REACTIVE OXYGEN ; CELLS ; IDENTIFICATION ; MACROPHAGES ; MECHANISMS ; COMPLEXITY ; DEFENSE
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000381537000053
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375579
专题	中国科学院大学
通讯作者	Song, Linsheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Shuai,Jia, Zhihao,Xin, Lusheng,et al. The cytochemical and ultrastructural characteristics of phagocytes in the pacific oyster crassostrea gigas[J]. Fish & shellfish immunology,2016,55:490-498.
APA	Jiang, Shuai.,Jia, Zhihao.,Xin, Lusheng.,Sun, Ying.,Zhang, Ran.,...&Song, Linsheng.(2016).The cytochemical and ultrastructural characteristics of phagocytes in the pacific oyster crassostrea gigas.Fish & shellfish immunology,55,490-498.
MLA	Jiang, Shuai,et al."The cytochemical and ultrastructural characteristics of phagocytes in the pacific oyster crassostrea gigas".Fish & shellfish immunology 55(2016):490-498.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。