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	Spycatcher-spytag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells
	Ke, Xianliang1; Zhang, Yuan2; Zheng, Fangliang3; Liu, Yan2; Zheng, Zhenhua2; Xu, Yi1; Wang, Hanzhong2
刊名	Chemical communications
	2018-02-04
卷号	54期号:10页码:1189-1192
ISSN号	1359-7345
DOI	10.1039/c7cc08880a
通讯作者	Zhang, yuan(zhangyuan@wh.iov.cn) ; Xu, yi(xuyi70@163.com)
英文摘要	A non-invasive labelling strategy is proposed to label baculovirus via genetic insertion of a spytag into the viral glycoprotein, followed by specific conjugation with the spycatcher protein on modified quantum dots (qds) through an isopeptide bond. the labelling method is convenient and efficient and shows little attenuation of viral infectivity. therefore, it is a biologically compatible technique for tracking viral infection.
WOS关键词	SINGLE-VIRUS TRACKING ; HOST-CELLS ; METABOLIC INCORPORATION ; ENVELOPED VIRUSES ; SPYTAG/SPYCATCHER ; COMPONENTS ; CHEMISTRY ; MEMBRANE ; PROTEIN
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000423784500007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2373342
专题	武汉病毒研究所
通讯作者	Zhang, Yuan; Xu, Yi
作者单位	1.Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Guangzhou 510623, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 3.Liaoning Univ, Sch Life Sci, Key Lab Anim Resource & Epidem Dis Prevent, Shenyang 110036, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Ke, Xianliang,Zhang, Yuan,Zheng, Fangliang,et al. Spycatcher-spytag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells[J]. Chemical communications,2018,54(10):1189-1192.
APA	Ke, Xianliang.,Zhang, Yuan.,Zheng, Fangliang.,Liu, Yan.,Zheng, Zhenhua.,...&Wang, Hanzhong.(2018).Spycatcher-spytag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells.Chemical communications,54(10),1189-1192.
MLA	Ke, Xianliang,et al."Spycatcher-spytag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells".Chemical communications 54.10(2018):1189-1192.

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