Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)

doi:10.1021/acs.jafc.7b02720

	Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)
	Li, Busu 1,2,3,4; Meng, Jie1,2,5 ; Li, Li 1,2,5; Liu, Sheng 1,2,3,4; Wang, Ting 1,2,3,4; Zhang, Guofan 1,2,4
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2017-09-06
卷号	65 期号:35 页码:7764-7773
关键词	Crassostrea Gigas Glycogenin Glycogen Biosynthesis Glycogen Synthase Alternative Splicing
DOI	10.1021/acs.jafc.7b02720
文献子类	Article
英文摘要	High glycogen levels in the Pacific oyster (Crassostrea gigas) contribute to its flavor, quality, and hardiness. Glycogenin (CgGN) is the priming glucosyltransferase that initiates glycogen biosynthesis. We characterized the full sequence and function of C. gigas CgGN. Three CgGN isoforms (CgGN-alpha, beta, and gamma) containing alternative exon regions were isolated. CgGN expression varied seasonally in the adductor muscle and gonadal area and was the highest in the adductor muscle. Autoglycosylation of CgGN can interact with glycogen synthase (CgGS) to complete glycogen synthesis. Subcellular localization analysis showed that CgGN isoforms and CgGS were located in the cytoplasm. Additionally, a site-directed mutagenesis experiment revealed that the Tyr200Phe and Tyr202Phe mutations could affect CgGN autoglycosylation. This is the first study of glycogenin function in marine bivalves. These findings will improve our understanding of glycogen synthesis and accumulation mechanisms in mollusks. The data are potentially useful for breeding high-glycogen oysters.
语种	英语
WOS记录号	WOS:000410255400020
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/143165]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266000, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Li, Busu,Meng, Jie,Li, Li,et al. Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(35):7764-7773.
APA	Li, Busu,Meng, Jie,Li, Li,Liu, Sheng,Wang, Ting,&Zhang, Guofan.(2017).Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas).JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(35),7764-7773.
MLA	Li, Busu,et al."Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.35(2017):7764-7773.