Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities

doi:10.1016/j.ijbiomac.2017.06.042

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	Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities
	Hou, Ningning1,2,3 ; Zhang, Meng 4,5; Xu, Yingjie5 ; Sun, Zhongmin 3,6; Wang, Jing1,3 ; Zhang, Lijuan 5; Zhang, Quanbin 1,3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2017-12-01
卷号	105 页码:1511-1518
关键词	Costaria Costata Polysaccharide Structure Anticoagulant Fibroblast Growth Factor (Fgf) Fibroblast Growth Factor Receptor (Fgfr)
DOI	10.1016/j.ijbiomac.2017.06.042
文献子类	Article
英文摘要	Crude polysaccharides from Costaria costata were extracted by hot water and further fractionated by anion exchange chromatography into three polysaccharide fractions. Three low molecular weight fragments were then prepared by degradation of the polysaccharides with hydrogen peroxide and ascorbic acid. The structural features of the polysaccharides and their low molecular weight fragments were elucidated for the first time based on the HGPC, FT-IR, NMR, MS, monosaccharide composition, and other chemical analyses. Their anticoagulant and FGF-1, -2, -7, -8, -9, -10/FGFR1c signaling activation activities in BaF3 cells were also examined. Our studies showed that the polysaccharides were sulfated at different positions of galactose and fucose residues. The APTT-, PT- and TT-based anticoagulant assay results indicated that a high molecular weight and a higher degree of sulfation were essential for their anticoagulant activities. In contrast, not only the polysaccharides but also the depolymerized fragments showed significant FGF/FGFR signal activating activities in a FGF-, molecular weight-, and sulfation-dependent manner. The results presented in current study demonstrated the potential use of the polysaccharides and their fragments as anticoagulants and FGF signal regulators. (C) 2017 Elsevier B.V. All rights reserved.
语种	英语
WOS记录号	WOS:000415779800019
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/143072]
专题	海洋研究所_海洋生物分类与系统演化实验室海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Tech, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China 5.Qingdao Univ, Affiliated Hosp, Inst Cerebrovasc Dis, Qingdao 266003, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Hou, Ningning,Zhang, Meng,Xu, Yingjie,et al. Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2017,105:1511-1518.
APA	Hou, Ningning.,Zhang, Meng.,Xu, Yingjie.,Sun, Zhongmin.,Wang, Jing.,...&Zhang, Quanbin.(2017).Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,105,1511-1518.
MLA	Hou, Ningning,et al."Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 105(2017):1511-1518.