Comparative proteomic profiles of the hepatopancreas in Fenneropenaeus chinensis response to hypoxic stress

doi:10.1002/pmic.200800518

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Comparative proteomic profiles of the hepatopancreas in Fenneropenaeus chinensis response to hypoxic stress
	Jiang, Hao 1,2; Li, Fuhua 1; Xie, Yusu 1,2; Huang, Bingxin 1,2; Zhang, Jinkang 1,2; Zhang, Jiquan 1; Zhang, Chengsong1,2 ; Li, Shihao 1,2; Xiang, Jianhai1
刊名	PROTEOMICS
	2009-06-01
卷号	9 期号:12 页码:3353-3367
关键词	2-de Chinese Shrimp Fenneropenaeus Chinensis Hypoxia Real-time Pcr
ISSN号	1615-9853
DOI	10.1002/pmic.200800518
文献子类	Review
英文摘要	Hypoxia, as one suboptimal environmental condition, can affect the physiological state of shrimp during pond aquaculture. To better understand the mechanism of response to hypoxic stress in Chinese shrimp Fenneropenaeus chinensis, proteome research approach was utilized. Differentially expressed proteins of hepatopancreas in adult Chinese shrimp between the control and hypoxia-stressed groups were screened. By 2-DE analysis, 67 spots showed obvious changes after hypoxia. Using LC-ESI-MS/MS, 51 spots representing 33 proteins were identified including preamylase, arginine kinase, phosphopyruvate hydratase, citrate synthase, ATP synthase alpha subunit, chymotrypsin BI, chitinase, ferritin, C-type lectin receptors, transketolase, formylglutathione hydrolase, formyltetrahydrofolate dehydrogenase, aldehyde dehydrogenase, glutathione peroxidase, cytosolic manganese superoxide dismutase, protein disulfide isomerase, beta-actin, oncoprotem nm23, crustacyanin-Cl and so on. These proteins could be functionally classified into several groups such as proteins related to energy production, metabolism-related proteins, immune-related proteins, antioxidant proteins, chaperones, cytoskeleton proteins and ungrouped proteins. The transcription levels of ten selected genes encode the identified proteins were analyzed by real-time PCR at different sampling times of hypoxia. This study is the first analysis of differentially expressed proteins in the hepatopancreas of shrimp after hypoxia and provides a new insight for further study in hypoxic stress response of shrimp at the protein level.; Hypoxia, as one suboptimal environmental condition, can affect the physiological state of shrimp during pond aquaculture. To better understand the mechanism of response to hypoxic stress in Chinese shrimp Fenneropenaeus chinensis, proteome research approach was utilized. Differentially expressed proteins of hepatopancreas in adult Chinese shrimp between the control and hypoxia-stressed groups were screened. By 2-DE analysis, 67 spots showed obvious changes after hypoxia. Using LC-ESI-MS/MS, 51 spots representing 33 proteins were identified including preamylase, arginine kinase, phosphopyruvate hydratase, citrate synthase, ATP synthase alpha subunit, chymotrypsin BI, chitinase, ferritin, C-type lectin receptors, transketolase, formylglutathione hydrolase, formyltetrahydrofolate dehydrogenase, aldehyde dehydrogenase, glutathione peroxidase, cytosolic manganese superoxide dismutase, protein disulfide isomerase, beta-actin, oncoprotem nm23, crustacyanin-Cl and so on. These proteins could be functionally classified into several groups such as proteins related to energy production, metabolism-related proteins, immune-related proteins, antioxidant proteins, chaperones, cytoskeleton proteins and ungrouped proteins. The transcription levels of ten selected genes encode the identified proteins were analyzed by real-time PCR at different sampling times of hypoxia. This study is the first analysis of differentially expressed proteins in the hepatopancreas of shrimp after hypoxia and provides a new insight for further study in hypoxic stress response of shrimp at the protein level.
学科主题	Biochemical Research Methods ; Biochemistry & Molecular Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000267793900011
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/2925]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Hao,Li, Fuhua,Xie, Yusu,et al. Comparative proteomic profiles of the hepatopancreas in Fenneropenaeus chinensis response to hypoxic stress[J]. PROTEOMICS,2009,9(12):3353-3367.
APA	Jiang, Hao.,Li, Fuhua.,Xie, Yusu.,Huang, Bingxin.,Zhang, Jinkang.,...&Xiang, Jianhai.(2009).Comparative proteomic profiles of the hepatopancreas in Fenneropenaeus chinensis response to hypoxic stress.PROTEOMICS,9(12),3353-3367.
MLA	Jiang, Hao,et al."Comparative proteomic profiles of the hepatopancreas in Fenneropenaeus chinensis response to hypoxic stress".PROTEOMICS 9.12(2009):3353-3367.