Diterpenoid compounds from Wedelia trilobata induce resistance to Tomato spotted wilt virus via the JA signal pathway in tobacco plants

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Diterpenoid compounds from Wedelia trilobata induce resistance to Tomato spotted wilt virus via the JA signal pathway in tobacco plants
	Zhao, Lihua 1; Hu, Zhonghui 2; Li, Shunlin 2; Zhou, Xueping 1,3; Li, Jing4 ; Su, Xiaoxia 1; Zhang, Lizhen 1; Zhang, Zhongkai 1; Dong, Jiahong 1
刊名	SCIENTIFIC REPORTS
	2019-02-26
卷号	9 页码:12
ISSN号	2045-2322
英文摘要	Tomato spotted wilt virus (TSWV) causes major losses of many crops worldwide. Several strategies have been attempted to control disease caused by TSWV. However, many challenges for the effective control of this disease remain. A promising approach is the use of abiotic or biotic inducers to enhance plant resistance to pathogens. We screened a diterpenoid compound from Wedelia trilobata, 3 alpha-Angeloyloxy-9 beta-hydroxy-ent-kaur-16-en-19-oic acid (AHK), which had higher curative and protective effects against TSWV than the ningnanmycin control. The rapid initiation of the expression of all the TSWV genes was delayed by more than 1d in the curative assay, and the expression of the NSs, NSm and RdRp genes was inhibited. In addition, the replication of all TSWV genes in systemic leaves was inhibited in the protective assay, with an inhibition rate of more than 90%. The concentrations of jasmonic acid (JA) and jasmonic acid isoleucine (JA-ILE) in the AHK-treated and systemic leaves of the treated plants were significantly higher than those observed in the control. The results suggested that AHK can induce systemic resistance in treated plants. The transcription of the NtCOI1 gene, a key gene in the JA pathway, was significantly higher in both the inoculated and systemic leaves of the AHK-treated plants compared to the control. The AHK-induced resistance to TSWV in Nicotiana benthamiana could be eliminated by VIGS-mediated silencing of the NtCOI1 gene. These results indicated that AHK can activate the JA pathway and induce systemic resistance to TSWV infection.
资助项目	Yunnan Provincial Top Scientists Input Program[2013HA028] ; National Natural Science Foundation of China[31470427] ; National Natural Science Foundation of China[31371919] ; Yunnan Provincial Natural Science Foundation[2017FA019] ; Yunnan Provincial Natural Science Foundation[2012CH007] ; Yunling Scholar Program
语种	英语
资助机构	Yunnan Provincial Top Scientists Input Program ; National Natural Science Foundation of China ; Yunnan Provincial Natural Science Foundation ; Yunling Scholar Program
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/64626]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Zhang, Zhongkai; Dong, Jiahong
作者单位	1.Minist Agr, Inst Biotechnol & Germplasm Resources, Key Lab Southwestern Crop Gene Resource & Germpla, Yunnan Acad Agr Sci,Yunnan Prov Key Lab Agr Biote, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 3.Zhejiang Univ, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China 4.Southwest Forestry Univ, Life Sci Coll, Kunming 650224, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Lihua,Hu, Zhonghui,Li, Shunlin,et al. Diterpenoid compounds from Wedelia trilobata induce resistance to Tomato spotted wilt virus via the JA signal pathway in tobacco plants[J]. SCIENTIFIC REPORTS,2019,9:12.
APA	Zhao, Lihua.,Hu, Zhonghui.,Li, Shunlin.,Zhou, Xueping.,Li, Jing.,...&Dong, Jiahong.(2019).Diterpenoid compounds from Wedelia trilobata induce resistance to Tomato spotted wilt virus via the JA signal pathway in tobacco plants.SCIENTIFIC REPORTS,9,12.
MLA	Zhao, Lihua,et al."Diterpenoid compounds from Wedelia trilobata induce resistance to Tomato spotted wilt virus via the JA signal pathway in tobacco plants".SCIENTIFIC REPORTS 9(2019):12.